A New Approach for Wounding Research: MYC2 Gene Expression and Protein Stability in Wounded Arabidopsis Protoplasts
Autor: | Miye Kwon, Seungmin Son, Jong Hee Im |
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Rok vydání: | 2021 |
Předmět: |
0106 biological sciences
0301 basic medicine mesophyll protoplast Cell Arabidopsis Plant Science Biology 01 natural sciences 03 medical and health sciences Protein stability LOX2 Downregulation and upregulation Gene expression medicine Kinase activity Transcription factor Ecology Evolution Behavior and Systematics integumentary system Ecology Communication fungi Botany Protoplast wounding biology.organism_classification Cell biology MYC2 030104 developmental biology medicine.anatomical_structure QK1-989 010606 plant biology & botany |
Zdroj: | Plants Plants, Vol 10, Iss 1518, p 1518 (2021) |
ISSN: | 2223-7747 |
Popis: | Wounding is a constant threat to plant survival throughout their lifespan; therefore, understanding the biological responses to wounds at the cellular level is important. The protoplast system is versatile for molecular biology, however, no wounding studies on this system have been reported. We established a new approach for wounding research using mechanically damaged Arabidopsis mesophyll protoplasts. Wounded protoplasts showed typical wounding responses, such as increased MPK6 kinase activity and upregulated JAZ1 expression. We also assessed expression profiles and protein stability of the basic helix-loop-helix transcription factor MYC2 in wounded protoplasts. Promoter activity, gene expression, and protein stability of MYC2 were compromised, but recovered in the early stage of wounding. In the late stage, the promoter activity and expression of MYC2 were increased, but the protein stability was not changed. According to the results of the present study, this new cell-based approach will be of use in various molecular studies on plant wounding. |
Databáze: | OpenAIRE |
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