An assay for the assessment of lipocortin 1 levels in human lung lavage fluid
| Autor: | S.F. Smith, N J Goulding, J L Godolphin, T.D. Tetley, Chris Roberts, Abraham Guz, R J Flower |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Lung Diseases
Hydrocortisone Sarcoidosis Annexins Coefficient of variation Blotting Western Immunology Inflammation Andrology Annexin medicine Humans Immunology and Allergy Lung Immunoassay medicine.diagnostic_test business.industry Calcium-Binding Proteins Respiratory disease medicine.disease Bronchoalveolar lavage medicine.anatomical_structure Cell culture medicine.symptom business Bronchoalveolar Lavage Fluid Glucocorticoid medicine.drug |
| Zdroj: | Journal of Immunological Methods. 131:119-125 |
| ISSN: | 0022-1759 |
| Popis: | The physiological function of the lipocortins, proteins which are thought to be glucocorticoid-regulated, is unclear. An improved assay for lipocortins might help to elucidate their role. A rapid and specific sandwich enzyme-linked immunosorbent assay (ELISA) for lipocortin 1 with a working range of 1-2000 ng/ml and an interrun coefficient of variation of less than 10% is described and used in this pilot study to quantify human lipocortin 1 for the first time in acellular bronchoalveolar lavage fluid (BALF), and in media conditioned by BAL cells, from control patients and those with pulmonary sarcoidosis. Using this assay a statistically significant relationship, not previously observed in man, has been demonstrated between concentrations of lipocortin 1/ml of BALF and serum cortisol levels (n = 10, rs = 0.6939, P less than 0.05). Although lipocortin 1 levels in acellular BALF were the same in control and sarcoid patients, significantly more lipocortin 1 was released from sarcoid BAL cells in culture (median 21.6, range 8.1-45.4 ng lipocortin/10(6) cells/h in culture) than from control cells (2.5, 1.5-7.6 ng lipocortin/10(6) cells/h in culture). The possible clinical significance of these data is discussed, but remains to be established. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|