Human papillomavirus 16 integration and risk factors associated in anal samples of HIV-1 infected men
| Autor: | María-Paz, Cañadas, Laila, Darwich, Guillermo, Sirera, Margarita, Bofill, Marta, Piñol, Francesc, Garcia-Cuyas, Mariona, Llatjos, Patricia, Corbasi, Bonaventura, Clotet, Sebastián, Videla, Eduard, Solaz |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Microbiology (medical)
Adult Male medicine.medical_specialty Multivariate analysis Virus Integration Anal Canal HIV Infections Dermatology Polymerase Chain Reaction Young Adult Risk Factors Internal medicine Cytology medicine Prevalence Humans Typing Risk factor Homosexuality Male Cervical cancer Human papillomavirus 16 business.industry Papillomavirus Infections Public Health Environmental and Occupational Health HPV infection virus diseases Middle Aged medicine.disease Anus Virology female genital diseases and pregnancy complications Infectious Diseases medicine.anatomical_structure Cross-Sectional Studies Spain Biomarker (medicine) RNA Viral business Precancerous Conditions |
| Zdroj: | Sexually transmitted diseases. 37(5) |
| ISSN: | 1537-4521 |
| Popis: | BACKGROUND The integration of HPV-16 DNA into the host genome is considered an important event in the progression of premalignant cervical lesions to cervical cancer. The aim of our study was to assess the prevalence of HPV-16 integration in anal cytologic specimens of HIV-1 infected men and its association with risk factors. PATIENTS METHODS: This cross-sectional study included 269 HIV-infected males. Detection and typing of HPV-infection was done by multiplex PCR, and integration of HPV-16 by real-time PCR. RESULTS The overall anal HPV-infection prevalence was 78% (209/269), 29% (77/269) for HPV-16 infection, and 9% (25/269) for HPV-16 integration. In HPV-16 infected group, the integration prevalence represented 32% (25/77). The only risk factor associated with HPV-16 integration was the time since HIV diagnosis (OR = 1.2, 95% CI: 1.0-1.3; P = 0.010). The risk factors associated with abnormal cytology results were: HPV infection (OR = 17.8, 95% CI: 6.8-46.6), HPV-16 infection (OR = 4.6, 95% CI: 2.5-8.4), and presence of HPV-16 integrated forms (OR = 11.7, 95% CI: 1.5-93.5). Moreover, in the multivariate analysis, the HPV-16 integration continued representing the most important risk factor (OR = 20, 95% CI: 1.6-226) for anal cytologic abnormalities. CONCLUSION HPV-16 infection and its integration in anal cells were highly prevalent in HIV-infected men. The assessment of HPV-16 integration rather than HPV-infection could be a good biomarker for predicting anal precancerous lesions in HIV-positive men. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|