Cell-free nucleic acids and melatonin levels in human follicular fluid predict embryo quality in patients undergoing in-vitro fertilization treatment

Autor: Ismail Abdur Rahman Khan Sherwani, Sana Abbas, Zaid Munir, Yousaf Latif Khan, Hikmet Hakan Aydin, Zahira Hassan, Shahzad Bhatti, Samina Suhail, Haroon Latif Khan
Přispěvatelé: Ege Üniversitesi
Jazyk: angličtina
Rok vydání: 2020
Předmět:
antral follicle count
Male
ovary follicle
Embryo quality
medicine.medical_treatment
cell free nucleic acid
Chorionic Gonadotropin
male infertility
thyrotropin
0302 clinical medicine
Ovarian Follicle
follitropin blood level
Pregnancy
Follicular phase
Prospective Studies
Melatonin
receiver operating characteristic
030219 obstetrics & reproductive medicine
predictive value
Estradiol
copy number variation
Obstetrics and Gynecology
clinical trial
Embryo
recombinant follitropin
transvaginal echography
chemiluminescence immunoassay
real time polymerase chain reaction
IVF
030220 oncology & carcinogenesis
mammalian embryo
luteinizing hormone
Circulating cell-free nucleic acids
blood sampling
tertiary care center
Female
follitropin
in vitro fertilization
Cell-Free Nucleic Acids
Infertility
Female
prospective study
medicine.drug
Adult
Infertility
DNA Copy Number Variations
female infertility
embryo
hormone blood level
gene dosage
DNA Fragmentation
Fertilization in Vitro
Follicular fluid
progesterone
purigon
chemistry
recombinant chorionic gonadotropin
Article
menstrual cycle
Andrology
03 medical and health sciences
Follicle
Ovulation Induction
blood
medicine
Humans
human
procedures
oocyte
Infertility
Male
embryo transfer
pregnancy outcome
In vitro fertilisation
ovary follicle fluid
business.industry
endometrial thickness
triptorelin
DNA
progesterone blood level
Embryo
Mammalian
medicine.disease
major clinical study
oocyte maturation
pregnant woman
thyrotropin blood level
ROC Curve
Reproductive Medicine
estradiol blood level
Oocytes
luteinizing hormone blood level
Follicle Stimulating Hormone
business
Muellerian inhibiting factor
Popis: Despite many advances in assisted reproductive technology (ART), the most viable embryo selection remains a challenge for infertility treatment. This study was designed to investigate whether intrafollicular circulating cell-free DNA (cfDNA) fragments and Melatonin levels predict embryo quality in patients undergoing IVF treatment. A total of eight hundred and ninety-five follicular fluid (ff) samples were collected from 325 infertile patients undergoing IVF treatment. Patients were enrolled from August 2017 to December 2018 in the infertility center of a tertiary care hospital. A clear non-hematic follicular fluid was aspirated after the removal of eggs from the dominant follicles (>18 mm) of each patient. Melatonin and E-2 levels in each follicular sample were estimated by immune-chemiluminescence using commercially available kits. ALU-qPCR evaluated cfDNA levels in individual follicular fluid samples. Our study presented a significant and negative relationship between intra-follicular cfDNA and melatonin concentration (-0.541: P = 25%) than embryos with low fragmentation rate (
Lahore institute of Fertility and Endocrinology, Hammed Latif Hospital
Lahore institute of Fertility and Endocrinology, Hammed Latif Hospital.
Databáze: OpenAIRE
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