Collective migration of an epithelial monolayer in response to a model wound
| Autor: | Pascal Silberzan, M. Poujade, A. Hertzog, Benoit Ladoux, J. Jouanneau, Philippe Chavrier, Erwan Grasland-Mongrain, A. Buguin |
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| Přispěvatelé: | Matière et Systèmes Complexes (MSC (UMR_7057)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Physico-Chimie Curie [Institut Curie] (PCC), Institut Curie [Paris]-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Compartimentation et dynamique cellulaires (CDC), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Curie [Paris]-Centre National de la Recherche Scientifique (CNRS), Matière et Systèmes Complexes (MSC), We gratefully acknowledge funding from the Ligue Nationale Contre le Cancer, the Fondation de France, and the Association pour la Recherche sur le Cancer., LabMSC, Directeur |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Cell signaling
Cell Culture Techniques Motility wound healing 02 engineering and technology Cell Communication Biology Models Biological Cell Line 03 medical and health sciences Dogs Cell Movement [SPI.MECA.BIOM] Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph] Cell polarity medicine Animals [PHYS.MECA.BIOM]Physics [physics]/Mechanics [physics]/Biomechanics [physics.med-ph] Autocrine signalling Cell damage Cell Shape Actin microfabrication 030304 developmental biology 0303 health sciences Multidisciplinary Hepatocyte Growth Factor [PHYS.MECA.BIOM] Physics [physics]/Mechanics [physics]/Biomechanics [physics.med-ph] Cell Polarity [SPI.MECA.BIOM]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph] Anatomy 021001 nanoscience & nanotechnology medicine.disease epithelial cells Microscopy Fluorescence Physical Sciences Commentary Biophysics Hepatocyte growth factor collective motility 0210 nano-technology Wound healing medicine.drug Signal Transduction |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2007, 104, pp.15988-15993 Proceedings of the National Academy of Sciences of the United States of America, 2007, 104 (41), pp.15988-15993. ⟨10.1073/pnas.0705062104⟩ |
| ISSN: | 0027-8424 1091-6490 |
| DOI: | 10.1073/pnas.0705062104⟩ |
| Popis: | Using an original microfabrication-based technique, we experimentally study situations in which a virgin surface is presented to a confluent epithelium with no damage made to the cells. Although inspired by wound-healing experiments, the situation is markedly different from classical scratch wounding because it focuses on the influence of the free surface and uncouples it from the other possible contributions such as cell damage and/or permeabilization. Dealing with Madin–Darby canine kidney cells on various surfaces, we found that a sudden release of the available surface is sufficient to trigger collective motility. This migration is independent of the proliferation of the cells that mainly takes place on the fraction of the surface initially covered. We find that this motility is characterized by a duality between collective and individual behaviors. On the one hand, the velocity fields within the monolayer are very long range and involve many cells in a coordinated way. On the other hand, we have identified very active “leader cells” that precede a small cohort and destabilize the border by a fingering instability. The sides of the fingers reveal a pluricellular actin “belt” that may be at the origin of a mechanical signaling between the leader and the followers. Experiments performed with autocrine cells constitutively expressing hepatocyte growth factor (HGF) or in the presence of exogenous HGF show a higher average velocity of the border and no leader. |
| Databáze: | OpenAIRE |
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