A Preliminary Evaluation of the Pro-Chondrogenic Potential of 3D-Bioprinted Poly(ester Urea) Scaffolds
| Autor: | Ana C. Fonseca, Bogdan Popa, Arménio C. Serra, Antonio Gloria, Ramaz Katsarava, Nigel M. Hooper, Miguel J. S. Ferreira, Samuel R. Moxon, Susan J. Kimber, Patricia dos Santos, David Tugushi, Marco Domingos |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Scaffold
Polymers and Plastics 0206 medical engineering Type II collagen 02 engineering and technology Article law.invention scaffold design lcsh:QD241-441 chemistry.chemical_compound lcsh:Organic chemistry Tissue engineering law Viability assay Aggrecan 3D bioprinting Chemistry General Chemistry 021001 nanoscience & nanotechnology Chondrogenesis 020601 biomedical engineering Cell biology poly(ester urea) tissue engineering Polycaprolactone cartilage repair poly(ester urea) scaffold design 0210 nano-technology |
| Zdroj: | Moxon, S R, Ferreira, M J S, Santos, P D, Popa, B, Gloria, A, Katsarava, R, Tugushi, D, Serra, A C, Hooper, N M, Kimber, S J, Fonseca, A C & Domingos, M A N 2020, ' A Preliminary Evaluation of the Pro-Chondrogenic Potential of 3D-Bioprinted Poly(ester Urea) Scaffolds ', Polymers, vol. 12, no. 7, pp. 1478 . https://doi.org/10.3390/polym12071478 Polymers (Basel) 12 (2020): 1–15. doi:10.3390/polym12071478 info:cnr-pdr/source/autori:Moxon S.R.; Ferreira M.J.S.; dos Santos P.; Popa B.; Gloria A.; Katsarava R.; Tugushi D.; Serra A.C.; Hooper N.M.; Kimber S.J.; Fonseca A.C.; Domingos M.A.N./titolo:A preliminary evaluation of the pro-chondrogenic potential of 3D-bioprinted poly(ester urea) scaffolds/doi:10.3390%2Fpolym12071478/rivista:Polymers (Basel)/anno:2020/pagina_da:1/pagina_a:15/intervallo_pagine:1–15/volume:12 Polymers Volume 12 Issue 7 Polymers, Vol 12, Iss 1478, p 1478 (2020) |
| DOI: | 10.3390/polym12071478 |
| Popis: | Degeneration of articular cartilage (AC) is a common healthcare issue that can result in significantly impaired function and mobility for affected patients. The avascular nature of the tissue strongly burdens its regenerative capacity contributing to the development of more serious conditions such as osteoarthritis. Recent advances in bioprinting have prompted the development of alternative tissue engineering therapies for the generation of AC. Particular interest has been dedicated to scaffold-based strategies where 3D substrates are used to guide cellular function and tissue ingrowth. Despite its extensive use in bioprinting, the application of polycaprolactone (PCL) in AC is, however, restricted by properties that inhibit pro-chondrogenic cell phenotypes. This study proposes the use of a new bioprintable poly(ester urea) (PEU) material as an alternative to PCL for the generation of an in vitro model of early chondrogenesis. The polymer was successfully printed into 3D constructs displaying adequate substrate stiffness and increased hydrophilicity compared to PCL. Human chondrocytes cultured on the scaffolds exhibited higher cell viability and improved chondrogenic phenotype with upregulation of genes associated with type II collagen and aggrecan synthesis. Bioprinted PEU scaffolds could, therefore, provide a potential platform for the fabrication of bespoke, pro-chondrogenic tissue engineering constructs. |
| Databáze: | OpenAIRE |
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