Graded Protein/PEG Nanopattern Arrays: Well-Defined Gradient Biomaterials to Induce Basic Cellular Behaviors
| Autor: | Junhu Zhang, Bai Yang, Hongchen Sun, Xingchi Chen, Wendong Liu, Zhanchen Cui, Zhongyi Gu, Jingjie Nan, Peihong Xue |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Materials science
Surface Properties Protein Array Analysis Biocompatible Materials Nanotechnology 02 engineering and technology 01 natural sciences Cell Line Polyethylene Glycols Mice chemistry.chemical_compound Cell Movement Etching (microfabrication) 0103 physical sciences PEG ratio Cell Adhesion Animals General Materials Science Well-defined Polarization (electrochemistry) Cell adhesion 010304 chemical physics Atom-transfer radical-polymerization technology industry and agriculture Substrate (chemistry) 021001 nanoscience & nanotechnology Extracellular Matrix chemistry 0210 nano-technology Ethylene glycol |
| Zdroj: | ACS Applied Materials & Interfaces. 11:1595-1603 |
| ISSN: | 1944-8252 1944-8244 |
| DOI: | 10.1021/acsami.8b16547 |
| Popis: | Gradient biomaterials have shown enormous potential in high-throughput screening of biomaterials and material-induced cell migration. To make the screening process more rapid and precise, improving the regularity of morphological structure and chemical modification on gradient biomaterials have attracted much attention. In this paper, we present a novel fabrication strategy to introduce ordered nanopattern arrays into gradient biomaterials, through combining surface-initiated atom transfer radical polymerization and inclined reactive-ion etching based on colloidal lithography. Graded protein/poly(ethylene glycol) (PEG) nanopattern arrays on a quartz substrate were fabricated and applied to affect the behaviors of cells. Owing to the continuously changed ratio of two different components, the corresponding cell adhesion density along the substrate showed obvious graded distribution after culturing for 24 h. Meanwhile, the cytoskeleton showed obvious polarization after culturing for 7 days, which is parallel with the direction of gradient. Additionally, oriented migration was generated when mouse MC3T3-E1 cells were cultured on the graded protein/PEG nanopattern arrays. On the basis of the ordered and well-defined nanopatterns, the correlation between the extracellular matrix and corresponding expressions generated by different stimuli can be investigated. |
| Databáze: | OpenAIRE |
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