Atrial natriuretic peptide inhibits iodide uptake and thyroglobulin messenger ribonucleic acid expression in cultured bovine thyroid follicles
| Autor: | A. H. Coleoni, Ana M. Cabanillas, Ana María Masini-Repiso, María Laura Vélez, Maria Eugenia Costamagna, Claudia Gabriela Pellizas |
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| Rok vydání: | 2002 |
| Předmět: |
medicine.medical_specialty
Thyroid Hormones Physiology medicine.medical_treatment Clinical Biochemistry Thyroid Gland chemistry.chemical_element Iodine Pertussis toxin Biochemistry Thyroglobulin Cellular and Molecular Neuroscience Endocrinology Atrial natriuretic peptide Internal medicine medicine Cyclic AMP Animals RNA Messenger Cyclic GMP Cells Cultured Thyroid Biological Transport Iodides medicine.anatomical_structure chemistry Gene Expression Regulation Pertussis Toxin cardiovascular system Cattle Cyclase activity hormones hormone substitutes and hormone antagonists Intracellular Atrial Natriuretic Factor circulatory and respiratory physiology Hormone Signal Transduction |
| Zdroj: | Regulatory peptides. 106(1-3) |
| ISSN: | 0167-0115 |
| Popis: | The involvement of atrial natriuretic peptide (ANP) in the regulation of thyroid gland is supported by the presence of high-affinity ANP receptors and the identification of the peptide in thyroid follicular cells. The aim of this work was to study the action of ANP on parameters of thyroid hormone biosynthesis and analyze the intracellular mechanism of the ANP action in cultured bovine thyroid follicles. The addition of ANP (0.1-10 nM) to the culture medium for 24 h inhibited the TSH (thyroid-stimulating hormone)-stimulated iodide uptake with a maximal inhibition at 1 nM ANP. When thyrocytes were incubated with 10 nM ANP the inhibitory effect slightly increased from 24 to 72 h. Thyroglobulin (Tg) mRNA expression was reduced by 1 and 10 nM ANP. After 24 h of treatment with the cGMP analogue, N(2),2'-O-dibutyrylguanosine 3':5'-cyclic monophosphate [(Bu)(2)cGMP] (0.1 and 1 mM), an inhibition of iodide uptake and Tg mRNA expression was obtained, evidencing a cGMP-mediated inhibitory signal in the thyroid cell. A reduction of the cAMP production was induced by incubation of thyroid follicles with 1 and 10 nM ANP for 24 h. Under a similar treatment the cGMP accumulation was increased only by 10 nM ANP. The inhibitory effect of ANP on Tg mRNA level was reverted in the presence of pertussis toxin, an inhibitor of the G(i)-protein-mediated reduction of the adenylate cyclase activity. These results indicate an inhibitory action of ANP on parameters of thyroid hormone biosynthesis. A G(i)-protein-mediated reduction of the cAMP production seems to be the main factor involved in the ANP action although a role of the cGMP pathway should not be discarded specially at high ANP levels. |
| Databáze: | OpenAIRE |
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