JASPer controls interphase histone H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila
| Autor: | Tom W. Muir, Peter B. Becker, Sylvain Maenner, Jørgen Johansen, Yeran Li, Catherine Regnard, Jack Girton, Kristen M. Johansen, Felix Wojcik, Tamas Schauer, Christian Albig, Geoffrey P. Dann, Weili Cai, Silke Krause, Chao Wang |
|---|---|
| Přispěvatelé: | Center for Integrated Protein Science (CIPSM), Technical University of Munich (TUM)-Helmholtz-Zentrum München (HZM)-Ludwig Maximilian University of Munich [Germany] (LMU München), Roy J Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University (ISU), Southwest Jiaotong University (SWJTU), Perelman School of Medicine, University of Pennsylvania [Philadelphia], Princeton University, Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM)-Ludwig Maximilian University of Munich [Germany] (LMU München)-Helmholtz-Zentrum München (HZM), Centre National de la Recherche Scientifique (CNRS)-Université de Lorraine (UL) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Heterochromatin Science [SDV]Life Sciences [q-bio] General Physics and Astronomy Kinases Protein Serine-Threonine Kinases Methylation Article Chromosomes General Biochemistry Genetics and Molecular Biology Cell Line Histones 03 medical and health sciences 0302 clinical medicine Histone post-translational modifications Animals Drosophila Proteins Humans Nucleosome Phosphorylation lcsh:Science Interphase Gene 030304 developmental biology 0303 health sciences Multidisciplinary biology Kinase Chemistry General Chemistry Chromatin Assembly and Disassembly Chromatin Nucleosomes Cell biology Drosophila melanogaster 030104 developmental biology Histone biology.protein lcsh:Q Heterochromatin protein 1 Protein Processing Post-Translational 030217 neurology & neurosurgery Post-translational modifications |
| Zdroj: | Nature Communications Nature Communications, Nature Publishing Group, 2019, 10 (1), pp.5343. ⟨10.1038/s41467-019-13174-6⟩ Nature Communications, Vol 10, Iss 1, Pp 1-17 (2019) |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-019-13174-6⟩ |
| Popis: | In flies, the chromosomal kinase JIL-1 is responsible for most interphase histone H3S10 phosphorylation and has been proposed to protect active chromatin from acquiring heterochromatic marks, such as dimethylated histone H3K9 (H3K9me2) and HP1. Here, we show that JIL-1’s targeting to chromatin depends on a PWWP domain-containing protein JASPer (JIL-1 Anchoring and Stabilizing Protein). JASPer-JIL-1 (JJ)-complex is the major form of kinase in vivo and is targeted to active genes and telomeric transposons via binding of the PWWP domain of JASPer to H3K36me3 nucleosomes, to modulate transcriptional output. JIL-1 and JJ-complex depletion in cycling cells lead to small changes in H3K9me2 distribution at active genes and telomeric transposons. Finally, we identify interactors of the endogenous JJ-complex and propose that JIL-1 not only prevents heterochromatin formation but also coordinates chromatin-based regulation in the transcribed part of the genome. The chromosomal kinase JIL-1 is responsible for interphase histone H3S10 phosphorylation and has been proposed to protect active chromatin from heterochromatinisation. Here, the authors show that JIL-1 is stabilized and anchored to active genes and telomeric transposons by JASPer, which binds to H3K36me3 nucleosomes via its PWWP domain. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|