Pharmacokinetic and brain distribution study of an anti‐glioblastoma agent in mice by HPLC–MS/MS

Autor:	Yaxin Li, Raina Dano, Cathy Li, Wenjing Zhang, Justin D. Lathia, Bingcheng Wang, Bin Su
Rok vydání:	2022
Předmět:	Pharmacology Clinical Biochemistry Administration Oral Reproducibility of Results General Medicine Biochemistry Article Analytical Chemistry Mice Blood-Brain Barrier Tandem Mass Spectrometry Drug Discovery Animals Humans Molecular Biology Chromatography High Pressure Liquid
Zdroj:	Biomed Chromatogr
ISSN:	1099-0801 0269-3879
DOI:	10.1002/bmc.5310
Popis:	Previously compound I showed great anti-glioblastoma activity without toxicity in a mouse xenograft study. In this study, a sensitive and rapid high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) method was developed and validated to investigate the pharmacokinetics and brain distribution of compound I in mice. The protein precipitation method was applied to extract the compound from mouse plasma and brain homogenates, and it was then separated using a Kinetex C(18) column with a mobile phase consisting of acetonitrile–0.1% formic acid water (50:50, v/v). The analytes were detected with multiple reaction monitoring for the quantitative response of the compounds. The inter- and intra-day precisions were
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::38743c0b84133676f561efb05286d41b https://doi.org/10.1002/bmc.5310 Zobrazit plný text záznamu Plný text