Chemical Fluorescent Probe for Detection of Aβ Oligomers
| Autor: | Srikanta Sahu, Young-Tae Chang, Seong-Wook Yun, Sunhee Cho, Dongdong Su, Sihyun Ham, Frances Prelli, Chai Lean Teoh, Eleanor Drummond, Sulgi Lim, Thomas Wisniewski |
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| Rok vydání: | 2015 |
| Předmět: |
Models
Molecular Amyloid Fibril Biochemistry Oligomer Article Catalysis Mice chemistry.chemical_compound Colloid and Surface Chemistry In vivo medicine Animals Fluorescent Dyes Amyloid beta-Peptides Chemistry Neurodegeneration Brain Fibrillogenesis General Chemistry medicine.disease Fluorescence In vitro Disease Models Animal Thermodynamics |
| Zdroj: | Journal of the American Chemical Society. 137:13503-13509 |
| ISSN: | 1520-5126 0002-7863 |
| DOI: | 10.1021/jacs.5b06190 |
| Popis: | Aggregation of amyloid β-peptide (Aβ) is implicated in the pathology of Alzheimer’s disease (AD), with the soluble, Aβ oligomeric species thought to be the critical pathological species. Identification and characterization of intermediate species formed during the aggregation process is crucial to the understanding of the mechanisms by which oligomeric species mediate neuronal toxicity and following disease progression. Probing these species proved to be extremely challenging, as evident by the lack of reliable sensors, due to their heterogeneous and transient nature. We describe here an oligomer-specific fluorescent chemical probe, BoDipy-Oligomer (BD-Oligo), developed through the use of the diversity-oriented fluorescent library approach (DOFLA) and high-content, imaging-based screening. This probe enables dynamic oligomer monitoring during fibrillogenesis in vitro and shows in vivo Aβ oligomers staining possibility in the AD mice model. |
| Databáze: | OpenAIRE |
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