Iron oxide core oil-in-water nanoemulsion as tracer for atherosclerosis MPI and MRI imaging

Autor: Tina Kauss, Gisèle Clofent-Sanchez, Mélusine Larivière, Julie Baillet, Sylvie Crauste-Manciet, Stéphane Mornet, Philippe Barthélémy, Alexandra Gaubert, Laurent Adumeau, Geoffrey Prévot, Marie Josée Jacobin-Valat, Cyril Lorenzato, Martine Duonor-Cérutti, Jeanny Laroche-Traineau
Přispěvatelé: Régulations Naturelles et Artificielles (ARNA), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Bordeaux Ségalen [Bordeaux 2], Centre de résonance magnétique des systèmes biologiques (CRMSB), Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB), Institut de Chimie de la Matière Condensée de Bordeaux (ICMCB), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Université de Bordeaux (UB), Baculovirus et Thérapie, Centre National de la Recherche Scientifique (CNRS), TRAIL program seeks to facilitate interdisciplinary, collaborative basic and clinical research in image based diagnostics and novel bio-compatible technologies for evaluating treatments, interventions and drug delivery strategies. Public grant from SVSE5 program. The microscopy was done in the Bordeaux Imaging Center, a service unit of the CNRS-INSERM and Bordeaux University, member of the national infrastructure France BioImaging., ANR-13-BSV5-0018,ATHERANOS,Théranostic de l'athérosclérose à l'aide de nanoparticules multi-modales fonctionnalisées avec des anticorps humains pour la délivrance de drogues in situ(2013), ANR-10-LABX-0053,MAbImprove,Optimization of therapeutic monoclonal antibodies development Better antibodies, better developed AND better used(2010), ANR-10-IDEX-0003,IDEX BORDEAUX,Initiative d'excellence de l'Université de Bordeaux(2010)
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: International Journal of Pharmaceutics
International Journal of Pharmaceutics, Elsevier, 2017, 532 (2), pp.669-676. ⟨10.1016/j.ijpharm.2017.09.010⟩
ISSN: 0378-5173
DOI: 10.1016/j.ijpharm.2017.09.010⟩
Popis: Purpose For early atherosclerosis imaging, magnetic oil-in-water nanoemulsion (NE) decorated with atheroma specific monoclonal antibody was designed for Magnetic Particle Imaging (MPI) and Magnetic Resonance Imaging (MRI). MPI is an emerging technique based on direct mapping of superparamagnetic nanoparticles which may advantageously complement MRI. Methods NE oily droplets were loaded with superparamagnetic iron oxide nanoparticles of 7, 11 and 18 nm and biofunctionalized with atheroma specific scFv-Fc TEG4-2C antibody. Results Inclusion of nanoparticles inside NE did not change the hydrodynamic diameter of the oil droplets, close to 180 nm, nor the polydispersity. The droplets were negatively charged (ζ = −30 mV). In vitro MPI signal was assessed by Magnetic Particle Spectroscopy (MPS). NE displayed MRI and MPS signals confirming its potential as new contrast agent. NE MPS signal increase with NPs size close to the gold standard (Resovist). In MRI, NE displayed R 2 * transversal relaxivity of 45.45, 96.04 and 218.81 mM −1 s −1 for 7, 11 and 18 nm respectively. NE selectively bind atheroma plaque both in vitro and ex vivo in animal models of atherosclerosis. Conclusion Magnetic NE showed reasonable MRI/MPS signals and a significant labelling of the atheroma plaque. These preliminary results support that NE platform could selectively image atherosclerosis.
Databáze: OpenAIRE
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