In-Depth Lipidomic Analysis of Molecular Species of Triacylglycerides, Diacylglycerides, Glycerophospholipids, and Sphingolipids of Buttermilk by GC-MS/FID, HPLC-ELSD, and UPLC-QToF-MS
| Autor: | Javier Fontecha, Pilar Castro-Gómez, Olimpio Montero |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Ceramide polar lipids Glycerophospholipids 01 natural sciences High-performance liquid chromatography Catalysis Article Mass Spectrometry Glycerides Inorganic Chemistry lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound Column chromatography Phosphatidylcholine Physical and Theoretical Chemistry Molecular Biology lcsh:QH301-705.5 Spectroscopy phospholipids chemistry.chemical_classification Phosphatidylethanolamine Flame Ionization 030109 nutrition & dietetics Chromatography sphingolipids 010401 analytical chemistry Organic Chemistry Fatty acid buttermilk General Medicine UPLC-QToF-MS 0104 chemical sciences Computer Science Applications chemistry Biochemistry lcsh:Biology (General) lcsh:QD1-999 lipids (amino acids peptides and proteins) Gas chromatography–mass spectrometry Chromatography Liquid |
| Zdroj: | International Journal of Molecular Sciences, Vol 18, Iss 3, p 605 (2017) International Journal of Molecular Sciences International Journal of Molecular Sciences; Volume 18; Issue 3; Pages: 605 |
| ISSN: | 1422-0067 |
| Popis: | Buttermilk, a byproduct of butter manufacturing, has gained considerable attention due to its high concentration of polar lipids as phospho- and sphingolipids from the milk fat globule membrane (MFGM). These polar lipids (PLs) are essential components of all cellular membranes and exert a variety of indispensable metabolic, neurological, and intracellular signaling processes. Despite its importance, there are few research studies that report a comprehensive characterization of the lipid molecular species of MFGM that could contribute to a better understanding of their putative healthful activities. In this study, procedures such as pressurized liquid extraction of polar and nonpolar lipids and their fractionation by flash chromatography have been carried out. The obtained fractions were submitted to an exhaustive characterization from a lipidomic point of view. The characterization includes new data about the identification and quantification of triacylglycerides (TAG), diacylglycerides (DAG), and phospho- and sphingolipids using different chromatographic techniques. The fatty acid profile was comparable to that of the milk fat but with a highly diverse composition of fatty acids. Molecular species have also been determined by using ultra-high performance liquid chromatography/quadruple-time-of-flight mass spectrometry (UPLC/QToF-MS). The TAG (16:0/16:0/6:0) and TAG (16:0/16:0/8:0) were the predominant saturated TAG species and TAG (14:0/18:1/16:0) and TAG (16:0/16:0/18:1) presented the highest content of monounsaturated TAG species. Furthermore; over 30 molecular species of phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), and phosphatidylinositol (PI) could be identified within PL, with PC (16:0/18:1) being the most abundant species. Whereas C16:0 was found to be the preferred FA in TAGs, it was C18:1 in PLs. Several ganglioside species have also been characterized with d18:1 ceramide moiety and secondary acyl chains ranging from C20:0 to C26:1. This approach could broaden the applications of high-resolution mass spectrometry for a better understanding of the role of MFGM and its functionality. |
| Databáze: | OpenAIRE |
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