Specific synbiotics in early life protect against diet-induced obesity in adult mice

Autor: Jan Knol, Fredrik Bäckhed, Tulika Arora, Eefje Engels, Kees van Limpt, Nina Sommer, Annemarie Oosting, Sebastian Tims, Mona Mischke, Annemarie M. Baars, Raish Oozeer
Rok vydání: 2017
Předmět:
0301 basic medicine
Blood Glucose
Male
Synbiotics
Endocrinology
Diabetes and Metabolism
Physiology
Gut flora
Bifidobacterium breve
Body composition
0302 clinical medicine
Endocrinology
Microbiologie
insulin resistance
Medicine
Glucose homeostasis
biology
Gastrointestinal Microbiome
Cholesterol
Phenotype
Liver
Female
Original Article
mouse model
030209 endocrinology & metabolism
liver
Microbiology
Mouse model
03 medical and health sciences
Insulin resistance
Ileum
dietary intervention
Internal Medicine
Animals
Microbiome
Obesity
VLAG
body composition
Obesity therapy
business.industry
Body Weight
Original Articles
medicine.disease
biology.organism_classification
Lipid Metabolism
Transplantation
Mice
Inbred C57BL
Dietary intervention
030104 developmental biology
Prebiotics
Diet
Western
obesity therapy
Body Constitution
Anti-Obesity Agents
business
Zdroj: Diabetes, Obesity & Metabolism
Diabetes, Obesity and Metabolism, 20(6), 1408-1418
Mischke, M, Arora, T, Tims, S, Engels, E, Sommer, N, van Limpt, K, Baars, A, Oozeer, R, Oosting, A, Bäckhed, F & Knol, J 2018, ' Specific synbiotics in early life protect against diet-induced obesity in adult mice ', Diabetes, Obesity and Metabolism, vol. 20, no. 6, pp. 1408-1418 . https://doi.org/10.1111/dom.13240
Diabetes, Obesity and Metabolism 20 (2018) 6
ISSN: 1463-1326
1462-8902
Popis: AIMS: The metabolic state of human adults is associated with their gut microbiome. The symbiosis between host and microbiome is initiated at birth, and early life microbiome perturbation can disturb health long-lastingly. Here, we determined how beneficial microbiome interventions in early life affect metabolic health in adulthood.METHODS: Postnatal diets were supplemented with either prebiotics (scGOS/lcFOS) or synbiotics (scGOS/lcFOS with Bifidobacterium breve M-16V) until post-natal (PN) day 42 in a well-established rodent model for nutritional programming. Mice were subsequently challenged with high-fat Western-style diet (WSD) for eight weeks. Body weight and composition were monitored, as was gut microbiota composition at PN21, 42 and 98. Markers of glucose homeostasis, lipid metabolism and host transcriptomics of six target tissues were determined in adulthood (PN98).RESULTS: Early life synbiotics protected mice against WSD-induced excessive fat accumulation throughout life, replicable in two independent European animal facilities. Adult insulin sensitivity and dyslipidemia were improved and most pronounced gene expression changes were observed in the ileum. We observed subtle changes in fecal microbiota composition, both in early life and in adulthood, including increased Bifidobacterium abundance. Microbiota transplantation using samples collected from synbiotics-supplemented adolescent mice at PN42 to age-matched germ-free recipients did not transfer the beneficial phenotype, indicating that synbiotics-modified microbiota at PN42 is not sufficient to transfer the long-lasting protection of the metabolic health status.CONCLUSION: Together, these findings show the potential and importance of timing of synbiotic interventions in early life during crucial microbiota development as preventive measure to ameliorate obesity risk and improve metabolic health throughout life.
Databáze: OpenAIRE
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