Minimally Invasive Approach to the Repair of Injured Skeletal Muscle With a Shape-memory Scaffold
| Autor: | Lan Cao, Zheng Wang, Janet Shansky, Herman H. Vandenburgh, Lin Wang, David J. Mooney |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A Muscle tissue Scaffold Soft Tissue Injuries Alginates Cell Survival Angiogenesis Myoblasts Skeletal Biocompatible Materials Context (language use) Mice chemistry.chemical_compound Glucuronic Acid Drug Discovery Genetics medicine Animals Myocyte Insulin-Like Growth Factor I Muscle Skeletal Molecular Biology Cell Proliferation Pharmacology Mice Inbred C3H Tissue Scaffolds business.industry Hexuronic Acids Regeneration (biology) Skeletal muscle Anatomy Cell biology Vascular endothelial growth factor Disease Models Animal medicine.anatomical_structure chemistry Molecular Medicine Original Article business |
| Zdroj: | Molecular Therapy. 22:1441-1449 |
| ISSN: | 1525-0016 |
| DOI: | 10.1038/mt.2014.78 |
| Popis: | Repair of injured skeletal muscle by cell therapies has been limited by poor survival of injected cells. Use of a carrier scaffold delivering cells locally, may enhance in vivo cell survival, and promote skeletal muscle regeneration. Biomaterial scaffolds are often implanted into muscle tissue through invasive surgeries, which can result in trauma that delays healing. Minimally invasive approaches to scaffold implantation are thought to minimize these adverse effects. This hypothesis was addressed in the context of a severe mouse skeletal muscle injury model. A degradable, shape-memory alginate scaffold that was highly porous and compressible was delivered by minimally invasive surgical techniques to injured tibialis anterior muscle. The scaffold controlled was quickly rehydrated in situ with autologous myoblasts and growth factors (either insulin-like growth factor-1 (IGF-1) alone or IGF-1 with vascular endothelial growth factor (VEGF)). The implanted scaffolds delivering myoblasts and IGF-1 significantly reduced scar formation, enhanced cell engraftment, and improved muscle contractile function. The addition of VEGF to the scaffold further improved functional recovery likely through increased angiogenesis. Thus, the delivery of myoblasts and dual local release of VEGF and IGF-1 from degradable scaffolds implanted through a minimally invasive procedure effectively promoted the functional regeneration of injured skeletal muscle. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|