High-throughput cellular-resolution synaptic connectivity mapping in vivo with concurrent two-photon optogenetics and volumetric Ca2+ imaging
| Autor: | Misha B. Ahrens, Minoru Koyama, Chao-Tsung Yang, Lixia Zhang, Dimitrii Tanese, Christopher McRaven, Valentina Emiliani |
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| Přispěvatelé: | Janelia Farm Research Campus, Howard Hughes Medical Institute (HHMI), Institut de la Vision, Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Tanese, Dimitrii, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
[PHYS.PHYS.PHYS-OPTICS] Physics [physics]/Physics [physics]/Optics [physics.optics]
[PHYS.PHYS.PHYS-OPTICS]Physics [physics]/Physics [physics]/Optics [physics.optics] 0303 health sciences [SPI.OPTI] Engineering Sciences [physics]/Optics / Photonic biology Computer science Optogenetics biology.organism_classification 3. Good health 03 medical and health sciences 0302 clinical medicine Neuronal circuits Cellular resolution Two-photon excitation microscopy In vivo [SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] Neuroscience Zebrafish 030217 neurology & neurosurgery 030304 developmental biology Ca2 imaging |
| DOI: | 10.1101/2020.02.21.959650 |
| Popis: | The ability to measure synaptic connectivity and properties is essential for understanding neuronal circuits. However, existing methods that allow such measurements at cellular resolution are laborious and technically demanding. Here, we describe a system that allows such measurements in a high-throughput way by combining two-photon optogenetics and volumetric Ca2+ imaging with whole-cell recording. We reveal a circuit motif for generating fast undulatory locomotion in zebrafish. |
| Databáze: | OpenAIRE |
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