Peculiarities of Immediate Response of Respiratory Chain Enzymes in Rat Cerebral Cortex to Hypoxia
| Autor: | Yu. I. Kirova, L. D. Luk'yanova, E. L. Germanova |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cytochrome Citric Acid Cycle Respiratory chain SDHA Mitochondrion General Biochemistry Genetics and Molecular Biology Electron Transport 03 medical and health sciences 0302 clinical medicine medicine Animals Hypoxia Cerebral Cortex Electron Transport Complex I biology Cytochrome b Chemistry General Medicine Hypoxia (medical) Electron transport chain Mitochondria Rats Cell biology 030104 developmental biology medicine.anatomical_structure Cerebral cortex biology.protein medicine.symptom Oxidation-Reduction 030217 neurology & neurosurgery |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 166:426-431 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/s10517-019-04365-8 |
| Popis: | We performed a complex study of the dependence of immediate reaction of catalytic subunits in mitochondrial enzymes (NDUFV2, SDHA, Cyt b, COX1, and ATP5A) in rat cerebral cortex (the most hypoxia-sensitive tissue) on the severity and duration of hypoxia in vivo and the role of individual resistance of rats to oxygen deficiency in this process. Three types of responses to hypoxia were revealed. The immediate response of mitochondria to oxygen deficiency appeared after its drop by 30-33% relatively to normal atmosphere level. It manifested in up-regulation of NAD-dependent oxidation, i.e., activation of respiratory chain complex I. Further decrease in oxygen concentration by 50% reprogrammed the work of respiratory chain via activation of respiratory chain complex II in parallel with down-regulation of the electron transport function of the respiratory chain complex I. This response was optimal for the expression of adaptation genes and for the formation of immediate tolerance of rats to hypoxia. The greatest drop of oxygen concentration by 60-62% reversed the Krebs cycle promoting recovery of the electron transport function of respiratory chain complex I. Despite this, the energy efficiency of the respiratory chain and the potency to mobilize the rapid adaptation mechanisms degraded due to abnormalities in cytochrome segment of the respiratory chain. |
| Databáze: | OpenAIRE |
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