Protamine precipitation of two reovirus particle types from polluted waters
| Autor: | R S Spendlove, D N Ridinger, D J Adams, B B Barnett |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1982 |
| Předmět: |
Protamine sulfate
viruses Sewage Reoviridae Fresh Water Applied Microbiology and Biotechnology Virus Microbiology Sonication medicine Chemical Precipitation Chymotrypsin Protamines Chromatography Ecology biology business.industry Precipitation (chemistry) Water Pollution biology.organism_classification Protamine biology.protein business Water Microbiology Fetal bovine serum Food Science Biotechnology medicine.drug Research Article |
| Popis: | Two forms of virus particle are released from reovirus-infected cell cultures, infectious reovirus and potentially infectious reovirus (PIV). PIV particle forms have a complete outer coat and are not infectious until the outer coat is altered or removed. The PIV concentration in polluted waters, however, has not been determined. Protamine sulfate precipitation, using 0.25% fetal bovine serum and 0.005% protamine sulfate for the first precipitation of the sample and 0.0025% for the second, was employed to concentrate infectious reovirus and PIV from water and sewage. Infectious reovirus and PIV particles were concentrated over 500-fold from river water inoculated with virus, and virus recoveries of between 80 and 100% were achieved. Virus precipitates stored at -20 degrees C as a protamine-virus concentrate showed a 5% loss of PIV after 14 days. Virus preparations were assayed, before and after treatment, with 200 micrograms of chymotrypsin per ml, using a fluorescent-antibody procedure. Protamine sulfate precipitation and fluorescent-antibody detection are effective ways to recover and assay reoviruses present in raw sewage. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|