Silk fibroin protein from mulberry and non-mulberry silkworms: cytotoxicity, biocompatibility and kinetics of L929 murine fibroblast adhesion
| Autor: | Subhas C. Kundu, Chitrangada Acharya, Sudip Ghosh |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Materials science
Biocompatibility Silk Biomedical Engineering Biophysics Fibroin Biocompatible Materials Bioengineering Nitric Oxide Cell Line Biomaterials Bombyx mori Polymer chemistry Antheraea mylitta Cell Adhesion Animals MTT assay Viability assay Cytotoxicity biology Tumor Necrosis Factor-alpha fungi Biomaterial Fibroblasts Bombyx Flow Cytometry biology.organism_classification Cell biology Macrophages Peritoneal Microscopy Electron Scanning Indicators and Reagents Fibroins |
| Zdroj: | Journal of Materials Science: Materials in Medicine. 19:2827-2836 |
| ISSN: | 1573-4838 0957-4530 |
| DOI: | 10.1007/s10856-008-3408-3 |
| Popis: | Silks fibers and films fabricated from fibroin protein of domesticated mulberry silkworm cocoon have been traditionally utilized as sutures in surgery and recently as biomaterial films respectively. Here, we explore the possibility of application of silk fibroin protein from non-mulberry silkworm cocoon as a potential biomaterial aid. In terms of direct inflammatory potential, fibroin proteins from Antheraea mylitta and Bombyx mori are immunologically inert and invoke minimal immune response. Stimulation of murine peritoneal macrophages and RAW 264.7 murine macrophages by these fibroin proteins both in solution and in the form of films assayed in terms of nitric oxide and TNFalpha production showed comparable stimulation as in collagen. Kinetics of adhesion of L929 murine fibroblasts, for biocompatibility evaluation, monitored every 4 h from seeding and studied over a period of 24 h, reveal A. mylitta fibroin film to be a better substrate in terms of rapid and easier cellularization. Cell viability studies by MTT assay and flow cytometric analyses indicate the ability of fibroin matrices to support cell growth and proliferation comparable to collagen for long-term culture. This matrix may have potential to serve in those injuries where rapid cellularization is essential. |
| Databáze: | OpenAIRE |
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