Autoproteolytic Fragments Are Intermediates in the Oligomerization/Aggregation of the Parkinson's Disease Protein Alpha-Synuclein as Revealed by Ion Mobility Mass Spectrometry

Autor:	John Rontree, Michael Przybylski, Christiaan Karreman, Karin M Danzer, Stefan Schildknecht, Nick Tomczyk, Michael L. Gross, Marcel Leist, James Langridge, Camelia Vlad, Thomas Ciossek, Bastian Hengerer, Kathrin Lindner, Alina Petre
Rok vydání:	2011
Předmět:	Spectrometry Mass Electrospray Ionization Ion-mobility spectrometry alpha-synuclein Electrospray ionization Proteolysis autoproteolytic fragmentation 010402 general chemistry Tandem mass spectrometry Mass spectrometry 01 natural sciences Biochemistry Mass Spectrometry Article oligomerization Aggregation 03 medical and health sciences chemistry.chemical_compound Biopolymers Tandem Mass Spectrometry Protein purification medicine Humans Molecular Biology Polyacrylamide gel electrophoresis Chromatography High Pressure Liquid mass spectrometry 030304 developmental biology Alpha-synuclein 0303 health sciences medicine.diagnostic_test Organic Chemistry Parkinson Disease 0104 chemical sciences chemistry ddc:540 alpha-Synuclein Molecular Medicine Electrophoresis Polyacrylamide Gel
Zdroj:	ChemBioChem. 12:2740-2744
ISSN:	1439-4227
Popis:	Gas-phase protein separation by ion mobility: With its ability to separate the Parkinson's disease protein Alpha-synuclein and its autoproteolytic products—despite the small concentrations of the latter—ion-mobility MS has enabled the characterization of intermediate fragments in in vitro oligomerization-aggregation. In particular, a possible key fragment, the highly aggregating C-terminal fragment, AlphaSyn(72–140), has been revealed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::379a907352208bfdb00ca2e2805af653 https://doi.org/10.1002/cbic.201100569 Zobrazit plný text záznamu Plný text