Genome sequence of Mycobacterium yongonense RT 955-2015 isolate from a patient misdiagnosed with multidrug-resistant tuberculosis: First clinical detection in Tanzania
| Autor: | Rudovick Kazwala, Dong-Jin Kim, Jongsik Chun, Sung-Min Ha, Esther Ngadaya, Pammla Petrucka, Kennedy Kwasi Addo, Nicholaus P. Mnyambwa, Sayoki Mfinanga |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Microbiology (medical) Tuberculosis Genotype Sequence analysis 030106 microbiology Biology Tanzania lcsh:Infectious and parasitic diseases 03 medical and health sciences Tuberculosis Multidrug-Resistant medicine Humans Mycobacterium yongonense lcsh:RC109-216 Diagnostic Errors Phylogeny Whole genome sequencing Mycobacterium avium complex (MAC) Whole Genome Sequencing Shotgun sequencing General Medicine Middle Aged Mycobacterium avium Complex 16S ribosomal RNA biology.organism_classification rpoB medicine.disease Multidrug-resistant tuberculosis (MDR-TB) Virology 3. Good health 030104 developmental biology Infectious Diseases Non-tuberculous mycobacteria (NTM) Mycobacterium |
| Zdroj: | International Journal of Infectious Diseases, Vol 71, Iss C, Pp 82-88 (2018) |
| ISSN: | 1878-3511 1201-9712 |
| Popis: | Background Mycobacterium yongonense is a recently described novel species belonging to Mycobacterium avium complex, which is the most prevalent aetiology of non-tuberculous mycobacteria associated with pulmonary infections, and poses tuberculosis diagnostic challenges in high-burden, resource-constrained settings. Methods Whole genome shotgun sequencing and comparative microbial genomic analyses were used to characterize the isolate from a patient diagnosed with multidrug-resistant tuberculosis (MDR-TB) after relapse. Results The genome sequence of the first case of M. yongonense ( M. yongonense RT 955-2015) in Tanzania is presented. Sequence analysis revealed that the RT 955-2015 strain had a high similarity to M. yongonense 05-1390(T) (98.74%) and Mycobacterium chimaera DSM 44623(T) (98%). Its 16S rRNA showed similarity to Mycobacterium paraintracellulare KCTC 290849(T) (100%), Mycobacterium intracellulare ATCC 13950(T) (100%), M. chimaera DSM 44623(T) (99.9%), and M. yongonense 05-1390(T) (98%). The strain exhibited a substantially different rpoB sequence to that of M. yongonense 05-1390 (95.16%), but closely related to that of M. chimaera DSM 44623(T) (99.86%), M. intracellulare ATCC 13950(T), (99.53%), and M. paraintracellulare KCTC 290849(T) (99.53%). Conclusions In light of the OrthoANI algorithm and phylogenetic analysis, it was concluded that the isolate was M. yongonense Type II genotype, which is an indication that the patient was misdiagnosed with TB/MDR-TB and received inappropriate treatment. |
| Databáze: | OpenAIRE |
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