A pumpless microfluidic device driven by surface tension for pancreatic islet analysis
| Autor: | Zequn Chen, James J. McGarrigle, Joshua E. Mendoza Elias, Yuan Xing, Jose Oberholzer, Mohammad Nourmohammadzadeh, Yong Wang, Manwai Chan |
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| Rok vydání: | 2016 |
| Předmět: |
Materials science
Microfluidics Biomedical Engineering 02 engineering and technology Fluorescent imaging 01 natural sciences Surface tension Islets of Langerhans Mice Lab-On-A-Chip Devices medicine Animals Humans Insulin Surface Tension Computer Simulation Insulin secretion Molecular Biology geography geography.geographical_feature_category Small volume Pancreatic islets 010401 analytical chemistry Optical Imaging Equipment Design 021001 nanoscience & nanotechnology Islet 0104 chemical sciences Transplantation medicine.anatomical_structure Hydrodynamics Stress Mechanical 0210 nano-technology Shear Strength Biomedical engineering |
| Zdroj: | Biomedical microdevices. 18(5) |
| ISSN: | 1572-8781 |
| Popis: | We present a novel pumpless microfluidic array driven by surface tension for studying the physiology of pancreatic islets of Langerhans. Efficient fluid flow in the array is achieved by surface tension-generated pressure as a result of inlet and outlet size differences. Flow properties are characterized in numerical simulation and further confirmed by experimental measurements. Using this device, we perform a set of biological assays, which include real-time fluorescent imaging and insulin secretion kinetics for both mouse and human islets. Our results demonstrate that this system not only drastically simplifies previously published experimental protocols for islet study by eliminating the need for external pumps/tubing and reducing the volume of solution consumption, but it also achieves a higher analytical spatiotemporal resolution due to efficient flow exchanges and the extremely small volume of solutions required. Overall, the microfluidic platform presented can be used as a potential powerful tool for understanding islet physiology, antidiabetic drug development, and islet transplantation. |
| Databáze: | OpenAIRE |
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