Characterization of macrophages from the bony fish gilthead seabream using an antibody against the macrophage colony-stimulating factor receptor
| Autor: | Iván Mulero, José Meseguer, Alfonsa García-Ayala, Victoriano Mulero, M. Pilar Sepulcre, Francisco J. Roca |
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| Rok vydání: | 2008 |
| Předmět: |
Macrophage colony-stimulating factor
Phagocyte Interleukin-1beta Molecular Sequence Data Immunology Receptor Macrophage Colony-Stimulating Factor Spleen Cell Separation Cross Reactions Antibodies Epitope Microbiology medicine Animals Macrophage Amino Acid Sequence Conserved Sequence Innate immune system biology Macrophages Sea Bream medicine.anatomical_structure Organ Specificity biology.protein Tumor necrosis factor alpha Antibody Sequence Alignment Granulocytes Developmental Biology |
| Zdroj: | Developmental & Comparative Immunology. 32:1151-1159 |
| ISSN: | 0145-305X |
| DOI: | 10.1016/j.dci.2008.03.005 |
| Popis: | Summary Two major professional phagocyte populations have been described in fish, namely granulocytes and monocytes/macrophages. Although the distribution and localization of macrophages have been documented in several teleost species using mainly light and/or electron microscopy, the lack of appropriate markers for these cells has hampered our in-depth knowledge of their biology. We report here the generation of a monospecific rabbit polyclonal antibody against the gilthead seabream macrophage colony-stimulating factor receptor (Mcsfr), which is an excellent marker of macrophages in mammals and the zebrafish. The anti-Mcsfr has been found to be very useful in immunohistochemistry (IHC) to specifically immunostain the purified macrophages (adherent cells) obtained from the head-kidney as well as different cell populations in paraffin-embedded organs, including the head-kidney, spleen, thymus, gills and liver. Unexpectedly, however, no Mcsfr immunoreactive (Mcsfr + ) cells were observed in the brain and intestine of the gilthead seabream. We also show that the distribution of Mcsfr + cells in the head-kidney and the spleen is unaltered following infection with the fish pathogenic bacterium Vibrio anguillarum and that the Il1b-producing cells in these two organs after infection are exclusively acidophilic granulocytes. Finally, as the epitope recognized by the anti-Mcsfr is well conserved, we illustrate the potential usefulness of this antibody in other teleost species, such as the European seabass. |
| Databáze: | OpenAIRE |
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