Naringin Ameliorates Monocrotaline-Induced Pulmonary Arterial Hypertension Through Endothelial-To-Mesenchymal Transition Inhibition
| Autor: | Yijia Xiang, Lingchun Lv, Changhong Cai, Huan Zhao, Yonghui Wu, Chunlai Zeng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
MAPK/ERK pathway RM1-950 030204 cardiovascular system & hematology Pharmacology Umbilical vein 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine pulmonary arterial hypertension medicine Pharmacology (medical) rat Naringin Original Research Lung naringin monocrotaline endothelial-to-mesenchymal transition Endothelial stem cell 030104 developmental biology Blood pressure medicine.anatomical_structure chemistry endothelial cell Therapeutics. Pharmacology Signal transduction Transforming growth factor |
| Zdroj: | Frontiers in Pharmacology, Vol 12 (2021) Frontiers in Pharmacology |
| ISSN: | 1663-9812 |
| DOI: | 10.3389/fphar.2021.696135/full |
| Popis: | Pulmonary arterial hypertension (PAH) caused by enhanced arterial pressure and increase vessel resistance in the lung. Endothelial-to-mesenchymal transition (EndMT) plays key roles in the vascular remodeling in PAH. Naringin, a protective gaseous mediator in PAH, is commonly extracted from tomatoes and citrus fruits (such as grapefruits), demonstrates effects in anti-inflammation, anti-oxidants, anti-proliferation and anti-tumor effects. Meanwhile, the association of Naringin and the process of EndMT is still unclear. In this study, monocrotaline (MCT) administration (60 mg/kg) was delivered for the induction of PAH in rats. Followed by that, Naringin (concentrations: 25, 50 and 100 mg/kg/day) were used for treatments. Human Umbilical Vein Endothelial Cells (HUVECs) were stimulated with Naringin and transforming growth factor β1 (TGFβ1, 10 ng/ml). As the result, Naringin was demonstrated to inhibit EndMT and alleviate PAH progression. In particular, HUVECs, Naringin significantly suppressed the mesenchymal marker expression induced by TGFβ1 treatment, enhanced the endothelial marker expression, and inhibited the activation of ERK and NF-κB signaling pathways. To conclude, this study provided novel evidences suggesting the beneficial effects of Naringin in PAH through the inhibition of the ERK and NF-κB signaling pathways and the EndMT progression in pulmonary arteries. |
| Databáze: | OpenAIRE |
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