JAM-A promotes wound healing by enhancing both homing and secretory activities of mesenchymal stem cells
| Autor: | Shizhao Ji, Zhaofan Xia, Yongjun Zheng, Houqi Liu, Shichu Xiao, Zhengdong Kong, He Fang, Kaihong Ji, Wu Minjuan, Yunqing Zhang |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Cell Mice Nude Receptors Cell Surface Biology Mice Cell Movement Cell Adhesion medicine Animals Humans Secretion Promoter Regions Genetic Transcription factor Mice Inbred BALB C Wound Healing integumentary system Cell adhesion molecule Chemotaxis Lentivirus Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells General Medicine Coculture Techniques Cell biology Platelet Endothelial Cell Adhesion Molecule-1 medicine.anatomical_structure Immunology Leukocytes Mononuclear Cytokine secretion Epidermis Wound healing Cell Adhesion Molecules Transcription Factors Homing (hematopoietic) |
| Zdroj: | Clinical Science. 129:575-588 |
| ISSN: | 1470-8736 0143-5221 |
| DOI: | 10.1042/cs20140735 |
| Popis: | The homing ability and secretory function of mesenchymal stem cells (MSCs) are key factors that influence cell involvement in wound repair. These factors are controlled by multilayer regulatory circuitry, including adhesion molecules, core transcription factors (TFs) and certain other regulators. However, the role of adhesion molecules in this regulatory circuitry and their underlying mechanism remain undefined. In the present paper, we demonstrate that an adhesion molecule, junction adhesion molecule A (JAM-A), may function as a key promoter molecule to regulate skin wound healing by MSCs. In in vivo experiments, we show that JAM-A up-regulation promoted both MSC homing to full-thickness skin wounds and wound healing-related cytokine secretion by MSCs. In vitro experiments also showed that JAM-A promoted MSC proliferation and migration by activating T-cell lymphoma invasion and metastasis 1 (Tiam1). We suggest that JAM-A up-regulation can increase the proliferation, cytokine secretion and wound-homing ability of MSCs, thus accelerating the repair rate of full-thickness skin defects. These results may provide insights into a novel and potentially effective approach to improve the efficacy of MSC treatment. |
| Databáze: | OpenAIRE |
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