The protective effects of fecal microbiota transplantation in an experimental model of necrotizing enterocolitis

Autor: Pricila Ávila, Monique Michels, Christian de Escobar Prado, Maria Vitoria Meller Milioli, Henrique Burger, Felipe Dal-Pizzol
Rok vydání: 2019
Předmět:
Zdroj: Journal of Pediatric Surgery. 54:1578-1583
ISSN: 0022-3468
DOI: 10.1016/j.jpedsurg.2018.10.045
Popis: Background Necrotizing enterocolitis (NEC) is a serious disease that affects premature neonates, causing high mortality. In the search for new options of treatment it was investigated whether fecal microbiota transplantation (FMT) decreased the inflammatory response during NEC development in experimental model. Methods Wistar rats were used and divided as follows: naive, control (NEC induction), FMT-before (transplantation of microbiota before insult) and FMT-after (microbiota transplantation after insult). The microbiota transplantation was performed by administering a feces solution obtained from an adult donor rat. The induction of enterocolitis involves feeding by artificial formula, hypothermia, hypoxia and endotoxin administration. MPO activity, TNF-α, IL-1β and IL-6 levels, oxidative and nitrosative damage and the grade of intestinal mucosa lesion were analyzed. Results The control group had a significant increase of inflammatory and oxidative parameters when compared to naive animals. Both FMT-before and after decreased all inflammatory and oxidative damage parameters when compared to control group. This was also true to the intestinal mucosa damage. Conclusion FMT administered just before or after NEC induction improved gut and systemic inflammation, and gut oxidative damage and intestinal injury.
Databáze: OpenAIRE
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