Effects of probiotic bacteria in dogs with food responsive diarrhoea treated with an elimination diet
| Autor: | Gloria Reuteler, Jalil Benyacoub, Christoph Cavadini, Karin Allenspach, Frédéric Gaschen, J.W. Blum, S.N. Sauter, Edgar Ontsouka, Ruth Knorr |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Diarrhea
Male medicine.medical_specialty Colon Duodenum media_common.quotation_subject Inflammatory bowel disease Gastroenterology Severity of Illness Index Microbiology law.invention Probiotic Feces Dogs Food Animals Double-Blind Method Enterobacteriaceae law Internal medicine Lactobacillus Elimination diet medicine Animals Dog Diseases Prospective Studies RNA Messenger Bifidobacterium media_common biology Probiotics Appetite medicine.disease biology.organism_classification Animal Feed Treatment Outcome Dietary Supplements Cytokines Animal Science and Zoology Animal Nutritional Physiological Phenomena Female Enterococcus Food Hypersensitivity |
| Zdroj: | Journal of animal physiology and animal nutrition. 90(7-8) |
| ISSN: | 0931-2439 |
| Popis: | We evaluated whether a probiotic supplementation in dogs with food responsive diarrhoea (FRD) has beneficial effects on intestinal cytokine patterns and on microbiota. Twenty-one client-owned dogs with FRD were presented for clinically needed duodeno- and colonoscopy and were enrolled in a prospective placebo (PL)-controlled probiotic trial. Intestinal tissue samples and faeces were collected during endoscopy. Intestinal mRNA abundance of interleukin (IL)-5, -10, -12p40 and -13, tumour necrosis factor-alpha, transforming growth factor-beta1 and interferon (IFN)-gamma were analysed and numbers of Lactobacillus spp., Bifidobacterium spp., Enterococcus spp. and Enterobacteriaceae and supplemented probiotic bacteria were determined in faeces. The Canine Inflammatory Bowel Disease Activity Index, a scoring system comprising general attitude, appetite, faecal consistency, defecation frequency, and vomitus, decreased in all dogs (p < 0.0001). Duodenal IL-10 mRNA levels decreased (p = 0.1) and colonic IFN-gamma mRNA levels increased (p = 0.08) after probiotic treatment. Numbers of Enterobacteriaceae decreased in FRD dogs receiving probiotic cocktail (FRD(PC)) and FRD dogs fed PL (FRD(PL)) during treatment (p < 0.05), numbers of Lactobacillus spp. increased in FRD(PC after) when compared with FRD(PC before) (p < 0.1). One strain of PC was detected in five of eight FRD(PC) dogs after probiotic supplementation. In conclusion, all dogs clinically improved after treatment, but cytokine patterns were not associated with the clinical features irrespective of the dietary supplementation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text