[6]-Gingerol-induced cell cycle arrest, reactive oxygen species generation, and disruption of mitochondrial membrane potential are associated with apoptosis in human gastric cancer (AGS) cells

Autor: Hannah R. Vasanthi, Veeresh Kumar Sali, Debjani P. Mansingh, O J Sunanda
Rok vydání: 2018
Předmět:
0301 basic medicine
Cell cycle checkpoint
Cell Survival
Health
Toxicology and Mutagenesis
Catechols
Apoptosis
Adenocarcinoma
Ginger
Toxicology
Biochemistry
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Downregulation and upregulation
Annexin
Stomach Neoplasms
Cell Line
Tumor
Ethidium
Humans
Annexin A5
Molecular Biology
Caspase
Chromatography
High Pressure Liquid
Cell Proliferation
bcl-2-Associated X Protein
chemistry.chemical_classification
Membrane Potential
Mitochondrial
Reactive oxygen species
biology
Chemistry
Plant Extracts
Acridine orange
Cytochromes c
General Medicine
Cell Cycle Checkpoints
Molecular biology
Acridine Orange
Up-Regulation
030104 developmental biology
Proto-Oncogene Proteins c-bcl-2
030220 oncology & carcinogenesis
Caspases
Cancer cell
biology.protein
Molecular Medicine
Fatty Alcohols
Reactive Oxygen Species
Protein Binding
Zdroj: Journal of biochemical and molecular toxicology. 32(10)
ISSN: 1099-0461
Popis: Ginger (Zingiber officinale Roscoe), a monocotyledonous herb, is widely used as an herbal medicine owing to the phytoconstituents it possesses. In the current study, the quantity of [6]-gingerol, the major phenolic ketone, in the fresh ginger and dried ginger rhizome was found to be 6.11 µg/mg and 0.407 µg/mg. Furthermore, [6]-gingerol was assessed for its antiapoptotic effects in human gastric adenocarcinoma (AGS) cells evidenced by acridine orange/ethidium bromide staining technique and Annexin-V assay. An increase in reactive oxygen species (ROS) generation led to a decrease in mitochondrial membrane potential (MMP) and subsequent induction of apoptosis. Results disclose that perturbations in MMP are associated with deregulation of Bax/Bcl-2 ratio at protein level, which leads to upregulation of cytochrome-c triggering the caspase cascade. These enduringly suggest that [6]-gingerol can be effectively used for targeting the mitochondrial energy metabolism to manage gastric cancer cells.
Databáze: OpenAIRE
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