Controlled packing and single-droplet resolution of 3D-printed functional synthetic tissues
| Autor: | Idil Cazimoglu, Ravinash Krishna Kumar, Matthew T. Cornall, Rebecca G. Allan, Carina Monico, Oliver J. Meacock, Hagan Bayley, Alessandro Alcinesio, Vanessa Restrepo Schild |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
endocrine system Fabrication Materials science Science Lipid Bilayers General Physics and Astronomy Bioengineering 02 engineering and technology complex mixtures Article General Biochemistry Genetics and Molecular Biology Contact angle Membrane biophysics Nanopores 03 medical and health sciences Biomimetic Materials Biomimetics lcsh:Science Total internal reflection Microscopy Confocal Multidisciplinary Tessellation Resolution (electron density) Temperature Bioinspired materials technology industry and agriculture Water General Chemistry 021001 nanoscience & nanotechnology Lipids eye diseases Kinetics Tissues Nanopore 030104 developmental biology Printing Three-Dimensional lcsh:Q 0210 nano-technology Biological system |
| Zdroj: | Nature Communications Nature Communications, Vol 11, Iss 1, Pp 1-13 (2020) |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-020-15953-y |
| Popis: | 3D-printing networks of droplets connected by interface bilayers are a powerful platform to build synthetic tissues in which functionality relies on precisely ordered structures. However, the structural precision and consistency in assembling these structures is currently limited, which restricts intricate designs and the complexity of functions performed by synthetic tissues. Here, we report that the equilibrium contact angle (θDIB) between a pair of droplets is a key parameter that dictates the tessellation and precise positioning of hundreds of picolitre-sized droplets within 3D-printed, multi-layer networks. When θDIB approximates the geometrically-derived critical angle (θc) of 35.3°, the resulting networks of droplets arrange in regular hexagonal close-packed (hcp) lattices with the least fraction of defects. With this improved control over droplet packing, we can 3D-print functional synthetic tissues with single-droplet-wide conductive pathways. Our new insights into 3D droplet packing permit the fabrication of complex synthetic tissues, where precisely positioned compartments perform coordinated tasks. Precise patterning of lipid-stabilised aqueous droplets is a key challenge in building synthetic tissue designs. Here, the authors show how the interactions between pairs of droplets direct the packing of droplets within 3D-printed networks, enabling the formation of synthetic tissues with high-resolution features. |
| Databáze: | OpenAIRE |
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