Physiological and Phylogenetic Characterization of Rhodotorula diobovata DSBCA06, a Nitrophilous Yeast
| Autor: | Manuela Pintus, Enrico Civiero, Francesca Sollai, Elena Tamburini, Enrico Sanjust, Paolo Zucca, Claudio Ruggeri |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Nitrogen assimilation chemistry.chemical_element Rhodotorula General Biochemistry Genetics and Molecular Biology Article nitrogen 03 medical and health sciences Ammonia chemistry.chemical_compound Bioremediation Nitrate bioremediation Nitrite nitrite lcsh:QH301-705.5 wastewater General Immunology and Microbiology biology biology.organism_classification Nitrogen 030104 developmental biology eutrophication chemistry lcsh:Biology (General) Environmental chemistry Nitrification General Agricultural and Biological Sciences |
| Zdroj: | Biology Volume 7 Issue 3 Biology, Vol 7, Iss 3, p 39 (2018) |
| ISSN: | 2079-7737 |
| Popis: | Agriculture and intensive farming methods are the greatest cause of nitrogen pollution. In particular, nitrification (the conversion of ammonia to nitrate) plays a role in global climate changes, affecting the bio-availability of nitrogen in soil and contributing to eutrophication. In this paper, the Rhodotorula diobovata DSBCA06 was investigated for growth kinetics on nitrite, nitrate, or ammonia as the sole nitrogen sources (10 mM). Complete nitrite removal was observed in 48 h up to 10 mM initial nitrite. Nitrogen was almost completely assimilated as organic matter (up to 90% using higher nitrite concentrations). The strain tolerates and efficiently assimilates nitrite at concentrations (up to 20 mM) higher than those previously reported in literature for other yeasts. The best growth conditions (50 mM buffer potassium phosphate pH 7, 20 g/L glucose as the sole carbon source, and 10 mM nitrite) were determined. In the perspective of applications in inorganic nitrogen removal, other metabolic features relevant for process optimization were also evaluated, including renewable sources and heavy metal tolerance. Molasses, corn, and soybean oils were good substrates, and cadmium and lead were well tolerated. Scale-up tests also revealed promising features for large-scale applications. Overall, presented results suggest applicability of nitrogen assimilation by Rhodotorula diobovata DSBCA06 as an innovative tool for bioremediation and treatment of wastewater effluents. |
| Databáze: | OpenAIRE |
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