Nuclear localization of the epstein-barr virus/c3d receptor (CR2) in the human burkitt b lymphoma cell, raji
| Autor: | Aline Gauffre, Raymond Frade, Jacques Hermann, Monique Barel, Edmond Puvion, Annie Viron |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Herpesvirus 4
Human Immunology chemical and pharmacologic phenomena Biology medicine.disease_cause Cell Line medicine Humans Nuclear pore Microscopy Immunoelectron Molecular Biology Cell Nucleus B-Lymphocytes Immunogold labelling Subcellular localization Nuclear matrix Burkitt Lymphoma Immunohistochemistry Virology Molecular biology Epstein–Barr virus Receptors Complement Raji cell Antigens Differentiation B-Lymphocyte Cell nucleus medicine.anatomical_structure Receptors Virus Receptors Complement 3d Nuclear localization sequence |
| Zdroj: | Molecular Immunology. 29:1113-1120 |
| ISSN: | 0161-5890 |
| DOI: | 10.1016/0161-5890(92)90044-x |
| Popis: | Epstein-Barr virus/C3d receptor (CR2) is a glycoprotein of mol. wt 140,000 expressed on the surface of Raji cells. We previously isolated phosphorylated CR2 from purified Raji cell nuclei. We have analyzed the nuclear localization of CR2 by electron microscope immunochemistry of thin sections of Raji cells and we have compared the binding properties of CR2 expressed on purified plasma membranes or nuclei. Anti-CR2 mAb immunogold labeling of thin sections of Raji cells identified CR2 at the nuclear surface and also within the nucleus. Nuclear envelope associated CR2 was localized mainly at nuclear pores. Within the nucleus, CR2 was associated with ribonucleoprotein (RNP) interchromatin fibrils. This labeling was preserved in nuclear matrix preparations. CR2 expressed on the surfaces of purified nuclei or on the cell surface interacted with soluble and particle-bound C3bi/C3d. Monoclonal anti-CR2 antibodies, which recognized extracellular domains of CR2, reacted differently with CR2 depending on its subcellular localization. The presence of CR2 in nuclei may be due to translocation of the cell surface CR2 and/or the presence of two distinct intracellular pathways for mature CR2. |
| Databáze: | OpenAIRE |
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