Red Light Activates the σB-Mediated General Stress Response of Bacillus subtilis via the Energy Branch of the Upstream Signaling Cascade▿
| Autor: | Marcela Avila-Perez, Jeroen B. van der Steen, Klaas J. Hellingwerf, Remco Kort |
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| Přispěvatelé: | Molecular Cell Physiology, AIMMS, Biophysics Photosynthesis/Energy, Molecular Microbial Physiology (SILS, FNWI) |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Phototropin
Light Phosphatase Sigma Factor Bacillus subtilis Biology Research Support Microbiology Downregulation and upregulation Bacterial Proteins Sigma factor Journal Article Non-U.S. Gov't Molecular Biology Oligonucleotide Array Sequence Analysis Research Support Non-U.S. Gov't fungi Bacterial Gene Expression Regulation Bacterial biology.organism_classification Deletion Mutagenesis Cell biology Regulon Gene Expression Regulation Signal transduction Signal Transduction |
| Zdroj: | Journal of Bacteriology, 192(3), 755-62. American Society for Microbiology Avila-Pérez, M, van der Steen, J B, Kort, R & Hellingwerf, K J 2010, ' Red light activates the sigmaB-mediated general stress response of Bacillus subtilis via the energy branch of the upstream signaling cascade ', Journal of Bacteriology, vol. 192, no. 3, pp. 755-62 . https://doi.org/10.1128/JB.00826-09 Journal of Bacteriology, 192(3), 755-762. American Society for Microbiology |
| ISSN: | 0021-9193 |
| DOI: | 10.1128/JB.00826-09 |
| Popis: | The σ B -dependent general stress response in the common soil bacterium Bacillus subtilis can be elicited by a range of stress factors, such as starvation or an ethanol, salt, or heat shock, via a complex upstream signaling cascade. Additionally, σ B can be activated by blue light via the phototropin homologue YtvA, a component of the environmental branch of the signaling cascade. Here we use a reporter-gene fusion to show that σ B can also be activated by red light via the energy branch of its upstream signaling cascade. Deletion mutagenesis and homologous overproduction experiments indicate that the RsbP protein (composed of an N-terminal Per-ARNT-Sim [PAS] domain and a C-terminal PP2C-type phosphatase domain) is involved in the red light response. This second light input pathway functions complementarily to YtvA; it shows broader spectral sensitivity but requires higher light intensities. These results are confirmed by transcriptome analyses, which show that both light effects result in upregulation of the σ B regulon, with minimal activation of other responses. |
| Databáze: | OpenAIRE |
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