Rapid Determination of Chlorophyll and Pheophytin in Green Tea Using Fourier Transform Infrared Spectroscopy
| Autor: | Yong He, Kaiwen Xu, Hui Fang, Zhou Ruiqing, Xiaoli Li, Juan-Juan Jin, Jie Xu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Pheophytin
Chlorophyll Infrared green tea pheophytin Analytical chemistry Pharmaceutical Science 01 natural sciences Article Analytical Chemistry lcsh:QD241-441 Chemometrics chemistry.chemical_compound symbols.namesake 0404 agricultural biotechnology lcsh:Organic chemistry Drug Discovery Spectroscopy Fourier Transform Infrared Physical and Theoretical Chemistry Fourier transform infrared spectroscopy Spectroscopy FT–IR spectroscopy Tea 010401 analytical chemistry Organic Chemistry Pheophytins 04 agricultural and veterinary sciences Green tea 040401 food science 0104 chemical sciences chlorophyll quantification determination Fourier transform chemistry Nonlinear Dynamics Chemistry (miscellaneous) symbols Molecular Medicine |
| Zdroj: | Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry Molecules; Volume 23; Issue 5; Pages: 1010 Molecules, Vol 23, Iss 5, p 1010 (2018) |
| ISSN: | 1420-3049 |
| Popis: | The chlorophyll, pheophytin, and their proportions are critical factors to evaluate the sensory quality of green tea. This research aims to establish an effective method to determine the quantification of chlorophyll and pheophytin in green tea, based on Fourier transform infrared (FT–IR) spectroscopy. First, five brands of tea were collected for spectral acquisition, and the chlorophyll and pheophytin were measured using the reference method. Then, a relation between these two pigments and FT–IR spectroscopy were developed based on chemometrics. Additionally, the characteristic IR wavenumbers of these pigments were extracted and proved to be effective for a quantitative determination. Successively, non-linear models were also built based on these characteristic wavenumbers, obtaining coefficients of determination of 0.87, 0.80, 0.85 and 0.89; and relative predictive deviations of 2.77, 2.62, 2.26 and 3.07 for the four pigments, respectively. These results demonstrate the feasibility of FT–IR spectroscopy for the determination of chlorophyll and pheophytin. |
| Databáze: | OpenAIRE |
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