The influence of sugar–protein complexes on the thermostability of C-reactive protein (CRP)
| Autor: | Diana Stan, Andreea Lorena Mateescu, Nicolae-Bogdan Mincu, Roxana Apetrei, Dana Stan, Bogdan Zorilă, Silvana Vasilca |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Models
Molecular 0301 basic medicine 030103 biophysics Macromolecular Substances Protein Conformation Molecular biology Science Molecular Conformation Inflammation Biochemistry Article Structure-Activity Relationship 03 medical and health sciences Medical research Spectroscopy Fourier Transform Infrared medicine Humans Sugar Thermostability Multidisciplinary biology Chemistry Biological techniques C-reactive protein Tryptophan C-Reactive Protein 030104 developmental biology biology.protein Biomarker (medicine) Medicine Steady state (chemistry) medicine.symptom Sugars Biomarkers Protein Binding Gamma irradiation |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | The purpose of the present study was to evaluate de influence of protein–sugar complexation on the stability and functionality of C-reactive protein, after exposure to constant high temperatures, in order to develop highly stable positive controls for in-vitro diagnostic tests. C-reactive protein is a plasmatic protein used as a biomarker for the diagnosis of a series of health problems such as ulcerative colitis, cardiovascular diseases, metabolic syndrome, due to its essential role in the evolution of chronic inflammation. The sugar–protein interaction was investigated using steady state and time resolved fluorescence. The results revealed that there are more than two classes of tryptophan, with different degree of accessibility for the quencher molecule. Our study also revealed that sugar–protein complexes have superior thermostability, especially after gamma irradiation at 2 kGy, the protein being stable and functional even after 22 days exposure to 40 °C. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|