Migration of monocytes across endothelium and passage through extracellular matrix involve separate molecular domains of PECAM-1
| Autor: | Tricia Greene, Hanh Huynh, Ana Eiroa, Elizabeth Polizzi, Fang Liao, William A. Muller |
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| Rok vydání: | 1995 |
| Předmět: |
Umbilical Veins
Endothelium Recombinant Fusion Proteins Molecular Sequence Data Immunology Antigens Differentiation Myelomonocytic Inflammation Biology Monocytes Immunoglobulin G Extracellular matrix Cell Movement Chlorocebus aethiops Cell Adhesion medicine Animals Humans Immunology and Allergy Cell adhesion Cells Cultured Base Sequence Cell adhesion molecule Monocyte Antibodies Monoclonal Articles Extracellular Matrix Protein Structure Tertiary Cell biology Platelet Endothelial Cell Adhesion Molecule-1 medicine.anatomical_structure cardiovascular system biology.protein Basal lamina Endothelium Vascular medicine.symptom Cell Adhesion Molecules |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| DOI: | 10.1084/jem.182.5.1337 |
| Popis: | During the inflammatory response, the adhesion molecule PECAM plays a crucial role in transendothelial migration, the passage of leukocytes across endothelium. We report here an additional role for PECAM in the subsequent migration of monocytes through the subendothelial extracellular matrix. PECAM has six immunoglobulin (Ig) superfamily domains. Monoclonal antibodies whose epitopes map to domains 1 and/or 2 selectively block monocyte migration through the endothelial junction, whereas those that map to domain 6 block only the migration through the extracellular matrix, trapping the monocyte between the endothelium and its basal lamina. Therefore, transendothelial migration (diapedesis) and passage through extracellular matrix (interstitial migration) are distinct and separable phases of monocyte emigration. Furthermore, distinct and separate Ig domains of PECAM are involved in mediating these two steps. |
| Databáze: | OpenAIRE |
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