Real-time detection of implant-associated neutrophil responses using a formyl peptide receptor-targeting NIR nanoprobe
| Autor: | Jun Zhou, Yi-Ting Tsai, Hong Weng, Ewin N Tang, Ashwin Nair, Dave Digant, Liping Tang |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Lipopolysaccharides
Prosthesis-Related Infections Materials science Neutrophils Biophysics Pharmaceutical Science Nanoprobe Bioengineering Inflammation 02 engineering and technology In Vitro Techniques Biomaterials Mice 03 medical and health sciences biocompatibility Computer Systems In vivo International Journal of Nanomedicine Drug Discovery medicine Animals Original Research 030304 developmental biology Mice Inbred BALB C 0303 health sciences Spectroscopy Near-Infrared Formyl peptide receptor Foreign-Body Reaction Organic Chemistry Prostheses and Implants General Medicine 021001 nanoscience & nanotechnology Receptors Formyl Peptide In vitro Nanostructures Cell biology Transplantation Nanomedicine Catheter-Related Infections Molecular Probes Immunology nanoprobe Female in vivo imaging medicine.symptom 0210 nano-technology Molecular probe Preclinical imaging |
| Zdroj: | International Journal of Nanomedicine |
| ISSN: | 1178-2013 |
| Popis: | Jun Zhou*, Yi-Ting Tsai*, Hong Weng, Ewin N Tang, Ashwin Nair, Digant P Davé, Liping Tang, Department of Bioengineering, University of Texas at Arlington, Arlington, TX *Both authors contributed equally to this workAbstract: Neutrophils play an important role in implant-mediated inflammation and infection. Unfortunately, current methods which monitor neutrophil activity, including enzyme measurements and histological evaluation, require many animals and cannot be used to accurately depict the dynamic cellular responses. To understand the neutrophil interactions around implant-mediated inflammation and infection it is critical to develop methods which can monitor in vivo cellular activity in real time. In this study, formyl peptide receptor (FPR)-targeting near-infrared nanoprobes were fabricated. This was accomplished by conjugating near-infrared dye with specific peptides having a high affinity to the FPRs present on activated neutrophils. The ability of FPR-targeting nanoprobes to detect and quantify activated neutrophils was assessed both in vitro and in vivo. As expected, FPR-targeting nanoprobes preferentially accumulated on activated neutrophils in vitro. Following transplantation, FPR-targeting nanoprobes preferentially accumulated at the biomaterial implantation site. Equally important, a strong relationship was observed between the extent of fluorescence intensity in vivo and the number of recruited neutrophils at the implantation site. Furthermore, FPR-targeting nanoprobes may be used to detect and quantify the number of neutrophils responding to a catheter-associated infection. The results show that FPR-targeting nanoprobes may serve as a powerful tool to monitor and measure the extent of neutrophil responses to biomaterial implants in vivo.Keywords: in vivo imaging, nanoprobe, neutrophils, inflammation, biocompatibility |
| Databáze: | OpenAIRE |
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