Protection Provided by an Encapsulated Live Attenuated ΔabcBA Strain of Brucella ovis against Experimental Challenge in a Murine Model
| Autor: | Teane M. A. Silva, Tatiane A. Paixão, Humberto M. Brandão, Luana C. A. Ximenes, Ana Patrícia C. Silva, Renato L. Santos, Auricélio A. Macêdo |
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| Rok vydání: | 2015 |
| Předmět: |
Microbiology (medical)
Male Brucella ovis Injections Subcutaneous Clinical Biochemistry Immunology Brucella Vaccine Biology Vaccines Attenuated Brucellosis Immunology and Allergy Animals Ovis Drug Carriers Mice Inbred BALB C Vaccines Vaccination Gene deletion Cells Immobilized biology.organism_classification Virology Bacterial Load Mice Inbred C57BL Disease Models Animal Liver Murine model Liver pathology Gene Deletion Injections Intraperitoneal Spleen Experimental challenge |
| Zdroj: | Clinical and vaccine immunology : CVI. 22(7) |
| ISSN: | 1556-679X |
| Popis: | This study aimed to evaluate theBrucella ovisΔabcBAstrain as a vaccine candidate in the murine model. BALB/c mice were subcutaneously or intraperitoneally immunized with a single dose or three doses of theB. ovisΔabcBAstrain and then were challenged with wild-typeB. ovis. Single or multiple immunizations provided only mild protection, with significantly smaller numbers of wild-typeB. ovisCFU in the livers of immunized mice but not in the spleens. Encapsulation ofB. ovisΔabcBAsignificantly improved protection against experimental challenges in both BALB/c and C57BL/6 mice. Furthermore, immunization with encapsulatedB. ovisΔabcBAmarkedly prevented lesions in the spleens and livers of experimentally challenged mice. These results demonstrated that the encapsulatedB. ovisΔabcBAstrain confers protection to mice; therefore, this strain has potential as a vaccine candidate for rams. |
| Databáze: | OpenAIRE |
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