Microtubules tune mechanosensitive cell responses
| Autor: | Shailaja Seetharaman, Batiste Boëda, Damarys Loew, Chiara De Pascalis, Stéphane Vassilopoulos, Benoit Vianay, Manuel Théry, Florent Dingli, Vanessa Roca, Sandrine Etienne-Manneville |
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| Přispěvatelé: | Polarité cellulaire, Migration et Cancer / Cell Polarity, Migration and Cancer, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Université Paris Descartes, Sorbonne Paris Cité, Institut Universitaire d'Hématologie (IUH), Université Paris Diderot - Paris 7 (UPD7), Institut National de la Santé et de la Recherche Médicale (INSERM), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Hopital Saint-Louis [AP-HP] (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut Curie, Research Center, PSL Research University, Laboratoire de Spectrométrie de Masse Protéomique, Institut Curie [Paris], Univ Paris 06, CNRS, Inst Myol, INSERM,UMR 974,FRE 3617, Paris, France, Partenaires INRAE |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
RHOA biology Chemistry macromolecular substances [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] Cell biology Focal adhesion 03 medical and health sciences Crosstalk (biology) 0302 clinical medicine Tubulin Microtubule biology.protein Mechanosensitive channels Mechanotransduction Cell adhesion 030217 neurology & neurosurgery 030304 developmental biology |
| Popis: | Mechanotransduction is a process by which cells sense the mechanical properties of their surrounding environment and adapt accordingly to perform cellular functions such as adhesion, migration and differentiation. Integrin-mediated focal adhesions are major sites of mechanotransduction and their connection with the actomyosin network is crucial for mechanosensing as well as the generation and transmission of forces onto the substrate. Despite having emerged as major regulators of cell adhesion and migration, the contribution of microtubules to mechanotransduction still remains elusive. Here, we show that actomyosin-dependent mechanosensing of substrate rigidity controls microtubule acetylation, a tubulin post-translational modification, by promoting the recruitment of the alpha-tubulin acetyl transferase (αTAT1) to focal adhesions. Microtubule acetylation, in turn, promotes GEF-H1 mediated RhoA activation, actomyosin contractility and traction forces. Our results reveal a fundamental crosstalk between microtubules and actin in mechanotransduction, which contributes to mechanosensitive cell adhesion and migration. |
| Databáze: | OpenAIRE |
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