High salt diet impairs dermal tissue remodeling in a mouse model of IMQ induced dermatitis
| Autor: | Péter Dobosy, Attila Szabo, Beáta Szebeni, Tivadar Tulassay, Domonkos Pap, Róbert István Agócs, Apor Veres-Székely, Lajos Kemény, Csenge Pajtók, Zoltán Veréb, István Németh, Ádám Vannay |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Physiology medicine.medical_treatment Dermatitis Sodium Chloride Extracellular matrix Animal Cells Cell Movement Immune Physiology Medicine and Health Sciences Immune Response Cells Cultured Connective Tissue Cells Innate Immune System Multidisciplinary PDGFB Imiquimod integumentary system Chemistry Software Engineering Animal Models Dermis Extracellular Matrix Cytokine medicine.anatomical_structure Experimental Organism Systems Connective Tissue Physical Sciences Cytokines Engineering and Technology Medicine medicine.symptom Cellular Types Anatomy Inflammation Mediators Research Article medicine.medical_specialty Computer and Information Sciences Immune Cells Science Immunology Motility Inflammation Mouse Models Research and Analysis Methods Peripheral blood mononuclear cell Computer Software Signs and Symptoms Model Organisms Internal medicine medicine Animals Humans Salt intake Sodium Chloride Dietary Fibroblast Nutrition Probiotics Body Weight Chemical Compounds Biology and Life Sciences Cell Biology Fibroblasts Molecular Development Diet Mice Inbred C57BL Disease Models Animal Endocrinology Biological Tissue Immune System Animal Studies Leukocytes Mononuclear Salts Clinical Medicine Biomarkers Developmental Biology |
| Zdroj: | PLoS ONE, Vol 16, Iss 11, p e0258502 (2021) PLoS ONE, Vol 16, Iss 11 (2021) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Recent animal studies, as well as quantitative sodium MRI observations on humans demonstrated that remarkable amounts of sodium can be stored in the skin. It is also known that excess sodium in the tissues leads to inflammation in various organs, but its role in dermal pathophysiology has not been elucidated. Therefore, our aim was to study the effect of dietary salt loading on inflammatory process and related extracellular matrix (ECM) remodeling in the skin. To investigate the effect of high salt consumption on inflammation and ECM production in the skin mice were kept on normal (NSD) or high salt (HSD) diet and then dermatitis was induced with imiquimod (IMQ) treatment. The effect of high salt concentration on dermal fibroblasts (DF) and peripheral blood mononuclear cells (PBMC) was also investigated in vitro. The HSD resulted in increased sodium content in the skin of mice. Inflammatory cytokine Il17 expression was elevated in the skin of HSD mice. Expression of anti-inflammatory Il10 and Il13 decreased in the skin of HSD or HSD IMQ mice. The fibroblast marker Acta2 and ECM component Fn and Col1a1 decreased in HSD IMQ mice. Expression of ECM remodeling related Pdgfb and activation phosphorylated (p)-SMAD2/3 was lower in HSD IMQ mice. In PBMCs, production of IL10, IL13 and PDGFB was reduced due to high salt loading. In cultured DFs high salt concentration resulted in decreased cell motility and ECM production, as well. Our results demonstrate that high dietary salt intake is associated with increased dermal pro-inflammatory status. Interestingly, although inflammation induces the synthesis of ECM in most organs, the expression of ECM decreased in the inflamed skin of mice on high salt diet. Our data suggest that salt intake may alter the process of skin remodeling. |
| Databáze: | OpenAIRE |
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