Increased epoxyeicosatrienoic acids and reduced soluble epoxide hydrolase expression in the preeclamptic placenta
| Autor: | Matteo Brunelli, Houli Jiang, Francesco Dalle Vedove, Gaetano Vattemi, Cristiano Fava, Valeria Guglielmi, Pietro Minuz, John Quilley, Giovanni Zanconato |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Erythrocytes Physiology soluble epoxide hydrolase Cytochrome P-450 CYP2J2 Umbilical cord Umbilical Cord CYP2J2 chemistry.chemical_compound 8 11 14-Eicosatrienoic Acid Cytochrome P-450 Enzyme System Pre-Eclampsia Pregnancy Medicine reproductive and urinary physiology Epoxide Hydrolases epoxyeicosatrienoic acids Immunohistochemistry medicine.anatomical_structure Biochemistry embryonic structures cardiovascular system Female lipids (amino acids peptides and proteins) Arachidonic acid Cytochrome P-450 CYP4A Cardiology and Cardiovascular Medicine Adult Epoxide hydrolase 2 medicine.medical_specialty hypertension placenta cytochrome P450 Article Preeclampsia Cytochrome P-450 CYP2C8 preeclampsia 03 medical and health sciences Fetus Internal medicine Placenta Internal Medicine Animals Humans cytochrome P450 epoxyeicosatrienoic acids hypertension placenta preeclampsia soluble epoxide hydrolase CYP2C8 business.industry medicine.disease 030104 developmental biology Endocrinology chemistry Case-Control Studies Eicosanoids Epoxy Compounds business |
| Popis: | BACKGROUND Epoxyeicosatrienoic acids (EETs) derived from cytochrome P450 (CYP)-dependent metabolism of arachidonic acid are increased in the plasma of women with preeclampsia as compared with normal pregnancy and are significantly higher in fetal than in maternal plasma and erythrocytes. We hypothesized that differences in EET synthesis or metabolism in the feto-placental unit contributed to the observed differences in circulating EETs. METHOD To evaluate EETs, formation as well as the expression of relevant CYP isoforms and the metabolizing enzyme, soluble epoxide hydrolase (sEH), biopsies of placenta were collected from 19 normal pregnancy and 10 preeclampsia at the time of cesarean section delivery. EETs were extracted from tissue homogenates and analyzed by liquid chromatography coupled with tandem mass spectrometry. RESULTS Both cis-EETs and trans-EETs were detected in the placenta. Concentration of total EETs was higher in the placenta from preeclampsia compared with normal pregnancy (2.37 ± 1.42 ng/mg vs. 1.20 ± 0.72 ng/mg, mean ± SD, P |
| Databáze: | OpenAIRE |
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