Bacteriocin formation by dominant aerobic sporeformers isolated from traditional maari
| Autor: | Hagrétou Sawadogo-Lingani, Bréhima Diawara, Mamoudou H. Dicko, Dennis Sandris Nielsen, Line Thorsen, Donatien Kabore, Mogens Jakobsen, Torben S. Berner |
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| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_treatment
Bacillus Bacillus subtilis Biology Peptides Cyclic Microbiology Adansonia Bacteriocins Bacteriocin Burkina Faso medicine Peptide sequence Spores Bacterial Protease Nucleic acid sequence General Medicine Antimicrobial Proteinase K biology.organism_classification Anti-Bacterial Agents Biochemistry Fermentation Seeds biology.protein Peptides Food Science |
| Zdroj: | International Journal of Food Microbiology. 154:10-18 |
| ISSN: | 0168-1605 |
| Popis: | The antimicrobial activity of 8 Bacillus spp. and 2 Lysinibacillus spp. representing the predominant aerobic sporeformers during traditional maari fermentations, a traditional fermented baobab seeds product from Burkina Faso, was investigated. The antimicrobial activity was assessed against a total of 31 indicator organisms representing various Gram-negative and positive pathogens. The screening showed that 3 Bacillus subtilis strains (B3, B122 and B222) in particular had antimicrobial activity against some Gram-positive organisms and were selected for further studies. It was found that the antimicrobial substances produced were heat stable, in-sensitive to catalase, sensitive to protease and trypsin but resistant to the proteolytic action of papain and proteinase K and equally active at pH values ranging from 3 to 11. Bacteriocin secretion started in late exponential growth phase and maximum activity was detected during the stationary growth phase. The production of bacteriocin by B. subtilis B3, B122 and B222 was dependent on the aeration conditions. Maximum production of bacteriocin was observed under reduced aeration. Specific primers were used to screen isolates B3, B122 and B222 for genes involved in the synthesis of the bacteriocins subtilosin A, subtilin, sublancin and ericin. Amplicons of the expected sizes were detected for iywB, sboA, sboX, albA and spaS involved in the biosynthesis of subtilosin and subtilin, respectively. The translated nucleotide sequences had 100% identity to the YiwB, SboX and SboA amino acid sequences of the subtilosin A producing B. subtilis subsp. subtilis strain 168. Interestingly there was a 3 amino acid deletion at the N-terminal part of AlbA in B3, B122 and B222 that probably alters the activity of this enzyme. Analysis of the spaS gene sequences of B3, B122 and B222, encoding a subtilin precursor peptide, showed that the translated nucleotide sequence had 98% identity with the corresponding SpaS amino acid sequence of subtilin producing B. subtilis subsp. spizizenii strain ATCC6633. |
| Databáze: | OpenAIRE |
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