Osteoconductive layer-by-layer films of Poly(4-hydroxy-L-proline ester) (PHPE) and Tannic acid
| Autor: | Salih Özçubukçu, Irem Erel-Goktepe, Sreeparna Banerjee, Bora Onat |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Polymers and Plastics Organic Chemistry Layer by layer General Physics and Astronomy 02 engineering and technology Polymer 021001 nanoscience & nanotechnology Bone tissue Extracellular matrix Polyester 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology medicine.anatomical_structure Chemical engineering chemistry Hyaluronic acid Tannic acid Bone cell Materials Chemistry medicine 0210 nano-technology |
| Zdroj: | European Polymer Journal. 103:101-115 |
| ISSN: | 0014-3057 |
| Popis: | Orthopedic implants have shown major success in clinics after successful orthopedic surgeries. However, they are also associated with several drawbacks such as local and systemic immune reaction in the body and improper integration of the bone with the implants. Osteoconductivity is a property of orthopedic implants that promotes bone cell adhesion and bone tissue integration on implant surfaces. This study presents use of a biodegradable cationic polyester, poly(4-hydroxy-L-proline ester) (PHPE) together with Tannic Acid (TA) as building blocks in construction of layer-by-layer (LbL) films to impart osteoconductive properties to multilayer films. Water-soluble complexes of PHPE and TA (PHPE-TA) were prepared at pH 4 and then LbL deposited at the surface without using a polymer counterpart. Multilayers were then cross-linked using NaIO4 to enhance their stability under physiological conditions. Potential of multilayers as an osteoconductive coating were assessed by (i) osteoblast-like cell adhesion; (ii) determination of collagen deposition by cells and (iii) the determination of extracellular matrix (ECM) mineralization and the results were compared to control substrates, i.e. cell culture plate well surface and collagen-coated substrates. PHPE-TA multilayers were found to be adhesive for SaOS-2 osteoblast-like cells and promoted collagen-rich nodule formation and mineralization of the ECM without causing cytotoxicity. PHPE-TA multilayers promoted higher alkaline phosphatase (ALP) activity and mineralization of the ECM, compared to collagen-coated surfaces. Importantly, PHPE-TA multilayers exhibited osteoconductive behavior without need for incorporation of bioactive natural polymers like collagen and hyaluronic acid or calcium phosphate ceramics into the multilayer films. Such LbL films are promising to modify the surfaces of orthopedic implants to impart osteoconductive properties to a surface. |
| Databáze: | OpenAIRE |
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