Evaluation of an ELISA to detect rabies antibodies in orally vaccinated foxes and raccoon dogs sampled in the field
| Autor: | Marine Wasniewski, J.L. Schereffer, K. Mahar, A.L. Guiot, L. Tribout, Florence Cliquet |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Estonia
Veterinary medicine Rabies Tetracycline Administration Oral Foxes Enzyme-Linked Immunosorbent Assay Biology Antibodies Viral Sensitivity and Specificity Serology Virology parasitic diseases medicine Animals Raccoon Dogs medicine.disease Vaccination Rabies Vaccines Vaccination Campaigns biology.protein France Antibody medicine.drug |
| Zdroj: | Journal of Virological Methods. 187:264-270 |
| ISSN: | 0166-0934 |
| DOI: | 10.1016/j.jviromet.2012.11.022 |
| Popis: | The assessment of the efficacy of oral vaccination in wildlife is based on detection in the teeth of a biomarker (tetracycline) which is incorporated in the vaccine bait, and the quantification of rabies antibodies. A blocking ELISA was evaluated and compared with the FAVN test and a validated in-house ELISA, using sera from foxes and raccoon dogs collected following oral vaccination campaigns in France and Estonia. Specificity reached 100% in sera from naive animals. A high concordance (95%) was observed between the BioPro ELISA and the FAVN test, which was similar in sera from red foxes and raccoon dogs. Concordance between the BioPro ELISA and the in-house ELISA reached 96.5% for sera from red foxes. The agreement with tetracycline results was excellent in the fox for both the BioPro ELISA (95.9%) and the FAVN test (91.8%). Concordance was slightly lower in the raccoon dog, with a value of 82.8% for the BioPro ELISA and 78.4% for the FAVN test. Rabies antibodies were detected with the BioPro ELISA in animals vaccinated with different types of vaccines and in highly haemolysed sera. The BioPro ELISA is a valuable test to assess the efficacy of oral vaccination in foxes and raccoon dogs. |
| Databáze: | OpenAIRE |
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