miRNA-187-5p Regulates Osteoblastic Differentiation of Bone Marrow Mesenchymal Stem Cells in Mice by Targeting ICAM1
| Autor: | Xinnan Ma, Jinglong Yan, Yutuo Fu, Guanghua Chen, Xin Wang, Yi Sun, Chao Feng, Chengchao Song |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Article Subject
General Biochemistry Genetics and Molecular Biology Mice Western blot Downregulation and upregulation stomatognathic system In vivo Osteogenesis microRNA medicine Animals Osteopontin Transcription factor Mice Inbred BALB C Mice Inbred C3H Osteoblasts General Immunology and Microbiology biology medicine.diagnostic_test Chemistry Cell Differentiation Mesenchymal Stem Cells General Medicine Alkaline Phosphatase Intercellular Adhesion Molecule-1 Cell biology Up-Regulation RUNX2 MicroRNAs Sp7 Transcription Factor biology.protein Medicine Alkaline phosphatase Osteoporosis Research Article |
| Zdroj: | BioMed Research International BioMed Research International, Vol 2020 (2020) |
| ISSN: | 2314-6141 2314-6133 |
| Popis: | Osteoporosis (OP) is a common bone metabolic disease, the process of which is fundamentally irreversible. Therefore, the investigation into osteoblastic differentiation of bone marrow mesenchymal stem cells (BMSCs) will provide more clues for OP treatment. In the present study, we found that microRNA-187-5p (miR-187-5p) played a key role on osteoblastic differentiation, which was significantly upregulated during osteogenic differentiation of BMSCs in mice. Moreover, overexpression of miR-187-5p suppressed osteoblastic differentiation of BMSCs through increasing alkaline phosphatase (ALP), matrix mineralization, and levels of Osterix (OSX), and osteopontin (OPN) as well as runt-related transcription factor 2 (Runx2) in vitro. The results in vivo indicated that the upregulation of miR-187-5p enhanced the efficacy of new bone formation in the heterotopic bone formation assay. Luciferase reporter assay and western blot analysis revealed that miR-187-5p was involved in osteogenesis by targeting intracellular adhesion molecule 1 (ICAM-1). Furthermore, ICAM-1 silence inhibited osteoblastic differentiation of BMSCs. Taken together, our results suggested for the first time that miR-187-5p may promote osteogenesis by targeting ICAM-1, and provided a possible therapeutic target for bone metabolic diseases. |
| Databáze: | OpenAIRE |
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