Antimalarial antisense activity of hexitol nucleic acids
| Autor: | Thomas S. Stewart, Piet Herdewijn, Arthur Van Aerschot, David Atkins, Maria Vega C. Flores |
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| Rok vydání: | 1999 |
| Předmět: |
Nuclease
General Veterinary biology Base Sequence Oligonucleotide Molecular Sequence Data Plasmodium falciparum Ribozyme General Medicine Oligonucleotides Antisense biology.organism_classification Antimalarials Infectious Diseases Biochemistry Insect Science Phosphodiester bond Nucleic acid biology.protein Animals Parasitology Antimalarial Agent Hexosediphosphates RNase H |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0932-0113 |
| Popis: | Antisense oligonucleotides and ribozymes have shown promise both as antimalarial agents and as tools for identifying genes vital for parasite survival. This task is urgent due to the ineffectiveness of current drug regimes on the most virulent human malarial parasite, Plasmodium falciparum. The development of new ways to modify and/or protect conventional phosphodiester oligonucleotides to improve nuclease resistance is also important. We assessed the effect of antisense oligonucleotides containing phosphorylated anhydrohexitols in suppressing the growth of P. falciparum in culture. The modified oligonucleotides were able to inhibit parasite growth in a sequence-specific manner, but not as well as the phosphorothioated antisense oligonucleotides, which are effective antimalarials at submicromolar concentrations. Two reasons are suggested: the absence of RNase H activation and differences in membrane transport. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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