Macrophage Serum-Based Adhesion to Plasma-Processed Surface Chemistry is Distinct from That Exhibited by Fibroblasts

Autor:	Galiya Sh. Malkov, David W. Grainger, Ellen R. Fisher, Marisha L. Godek
Rok vydání:	2007
Předmět:	Polymers and Plastics Chemistry Cell growth Monocyte Cell Adhesion Condensed Matter Physics Molecular biology Article medicine.anatomical_structure Biochemistry Cell culture medicine Macrophage Cell adhesion Fibroblast
Zdroj:	Plasma processes and polymers (Print). 3(6-7)
ISSN:	1612-8850
Popis:	Plasma-polymerized films deposited from AlAm, HxAm, NVP, NVFA, AA and FC were compared to TCPS and PS surfaces in supporting cellular attachment, viability, and proliferation in serum-based culture in vitro for extended periods of time (>7 d). Surface patterns were created using multi-step depositions with physical masks. Cell adhesion in the presence of serum was compared for (monocyte-) macrophage and fibroblast cell lines. Cellular response was tracked over time, reporting adhesive behavior, proliferative rates, and morphological changes as a function of surface chemistry. Micropatterned surfaces containing different surface chemistries and functional groups (e.g. -NH(2), -COOH, -CF(3)) produced differential cell adhesive patterns for NIH 3T3 fibroblasts compared to J774A.1, RAW 264.7 or IC-21 (monocyte-) macrophage cell types. Significantly, macrophage adhesion is substantial on surfaces where fibroblasts do not adhere under identical culture conditions.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::35d5a6272c4e3201d22bae22dd033775 https://pubmed.ncbi.nlm.nih.gov/17417668 Zobrazit plný text záznamu Plný text