Genes regulating gland development in the cotton plant
| Autor: | LeAnne M. Campbell, Lorraine S. Puckhaber, Madhusudhana R. Janga, Stephany Toinga Villafuerte, Alan E. Pepper, Keerti S. Rathore, Robert D. Stipanovic, Jodi A. Scheffler, Devendra Pandeya, Kranti Konganti |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Transposable element Plant Science Biology 01 natural sciences 03 medical and health sciences chemistry.chemical_compound VIGS cottonseed Transcription (biology) Gene Expression Regulation Plant comparative transcriptomics transcription factors Gene expression Coding region Gene silencing Gene CRISPR/Cas9 Research Articles protein source Genetics Gossypium Gossypol cotton (Gossypium hirsutum) Phenotype Plant Leaves 030104 developmental biology chemistry Seeds glanding nutrition security RNA‐seq Agronomy and Crop Science 010606 plant biology & botany Biotechnology Research Article |
| Zdroj: | Plant Biotechnology Journal |
| ISSN: | 1467-7652 |
| Popis: | Summary In seeds and other parts of cultivated, tetraploid cotton (Gossypium hirsutum L.), multicellular groups of cells lysigenously form dark glands containing toxic terpenoids such as gossypol that defend the plant against pests and pathogens. Using RNA‐seq analysis of embryos from near‐isogenic glanded (Gl 2 Gl 2 Gl 3 Gl 3) versus glandless (gl 2 gl 2 gl 3 gl 3) plants, we identified 33 genes that expressed exclusively or at higher levels in embryos just prior to gland formation in glanded plants. Virus‐induced gene silencing against three gene pairs led to significant reductions in the number of glands in the leaves, and significantly lower levels of gossypol and related terpenoids. These genes encode transcription factors and have been designated the ‘Cotton Gland Formation’ (CGF) genes. No sequence differences were found between glanded and glandless cotton for CGF1 and CGF2 gene pairs. The glandless cotton has a transposon insertion within the coding sequence of the GoPGF (synonym CGF3) gene of the A subgenome and extensive mutations in the promoter of D subgenome homeolog. Overexpression of GoPGF (synonym CGF3) led to a dramatic increase in gossypol and related terpenoids in cultured cells, whereas CRISPR/Cas9 knockout of GoPGF (synonym CGF3) genes resulted in glandless phenotype. Taken collectively, the results show that the GoPGF (synonym CGF3) gene plays a critical role in the formation of glands in the cotton plant. Seed‐specific silencing of CGF genes, either individually or in combination, could eliminate glands, thus gossypol, from the cottonseed to render it safe as food or feed for monogastrics. |
| Databáze: | OpenAIRE |
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