Effects of neo-adjuvant chemotherapy for oesophago-gastric cancer on neuro-muscular gastric function
| Autor: | Steve J. Goodyear, E. Z. H. Sung, David Snead, Ramesh P. Arasaradnam, Chuka U. Nwokolo, Richard A. Borman, Gareth J. Sanger, Sean James, Emma M. Jarvie |
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| Rok vydání: | 2012 |
| Předmět: |
Male
RM medicine.medical_specialty Carbachol Esophageal Neoplasms medicine.medical_treatment Adenocarcinoma Cholinergic Agonists In Vitro Techniques Deoxycytidine Motilin RC0254 Contractility Stomach Neoplasms Internal medicine Antineoplastic Combined Chemotherapy Protocols Genetics medicine Humans Molecular Biology Ganglia Autonomic Capecitabine Aged Epirubicin Cisplatin Chemotherapy Gastric emptying business.industry Stomach digestive oral and skin physiology Muscle Smooth General Medicine Middle Aged Neoadjuvant Therapy Endocrinology medicine.anatomical_structure Gastric Emptying Chemotherapy Adjuvant Gastric Mucosa Acetylcholinesterase Ghrelin Female Fluorouracil business medicine.drug Muscle Contraction |
| Zdroj: | Molecular biology reports. 39(12) |
| ISSN: | 1573-4978 0301-4851 |
| Popis: | Delayed gastric emptying symptoms are often reported after chemotherapy. This study aims to characterise the effects of chemotherapy on gastric neuro-muscular function. Patients undergoing elective surgery for oesophago-gastric cancer were recruited. Acetylcholinesterase, nNOS, ghrelin receptor and motilin expressions were studied in gastric sections from patients receiving no chemotherapy (n = 3) or oesophageal (n = 2) or gastric (n = 2) chemotherapy. A scoring system quantified staining intensity (0–3; no staining to strong). Stomach sections were separately suspended in tissue baths for electrical field stimulation (EFS) and exposure to erythromycin or carbachol; three patients had no chemotherapy; four completed cisplatin-based chemotherapy within 6 weeks prior to surgery. AChE expression was markedly decreased after chemotherapy (scores 2.3 ± 0.7, 0.5 ± 0.2 and 0 ± 0 in non-chemotherapy, oesophageal- and gastric-chemotherapy groups (p < 0.03 each) respectively. Ghrelin receptor and motilin expression tended to increase (ghrelin: 0.7 ± 0.4 vs 2.0 ± 0.4 and 1.2 ± 0.2 respectively; p = 0.04 and p = 0.2; motilin: 0.7 ± 0.5 vs 2.2 ± 0.5 and 2.0 ± 0.7; p = 0.06 and p = 0.16). Maximal contraction to carbachol was 3.7 ± 0.7 g and 1.9 ± 0.8 g (longitudinal muscle) and 3.4 ± 0.4 g and 1.6 ± 0.6 (circular) in non-chemotherapy and chemotherapy tissues respectively (p < 0.05 each). There were loss of AChE and reduction in contractility to carbachol. The tendency for ghrelin receptors to increase suggests an attempt to upregulate compensating systems. Our study offers a mechanism by which chemotherapy markedly alters neuro-muscular gastric function. |
| Databáze: | OpenAIRE |
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