Role of chemical crosslinking in material-driven assembly of fibronectin (nano)networks: 2D surfaces and 3D scaffolds

Autor: Luis Gomez-Estrada, Roser Sabater i Serra, Manuel Salmerón-Sánchez, Antonio Sánchez-Laosa, José Luis Gómez Ribelles, Gloria Gallego Ferrer, Laia León-Boigues
Rok vydání: 2016
Předmět:
3D-fibrillogenesis
Ethylene glycol dimethacrylate
Supramolecular chemistry
02 engineering and technology
010402 general chemistry
Fibril
Electron Microscopy Service of the UPV
01 natural sciences
Article
chemistry.chemical_compound
Colloid and Surface Chemistry
Adsorption
Polymer chemistry
Physical and Theoretical Chemistry
Fibronectin
ComputingMethodologies_COMPUTERGRAPHICS
Scaffolds
chemistry.chemical_classification
Calorimetry
Differential Scanning
Fibrillogenesis
Surfaces and Interfaces
General Medicine
Polymer
021001 nanoscience & nanotechnology
Poly(ethyl acrylate)
Fibronectins
0104 chemical sciences
Cross-Linking Reagents
Bioactive substrates
chemistry
Chemical engineering
FISICA APLICADA
Thermogravimetry
MAQUINAS Y MOTORES TERMICOS
INGENIERIA ELECTRICA
Ethyl acrylate
0210 nano-technology
Glass transition
Crosslinked network
Biotechnology
Zdroj: RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname
Colloids and Surfaces. B, Biointerfaces
ISSN: 0927-7765
Popis: Graphical abstract
Highlights • Poly(ethyl acrylate) crosslinking alters adsorbed fibronectin (FN) organization. • FN fibrillogenesis induced by poly(ethyl acrylate) is kept up to 2% of crosslinker. • Adsorbed FN on scaffolds showed physiological-like nano(networks). • FN fibrillogenesis induced by poly(ethyl acrylate) was proved in 3D environments.
Poly(ethyl acrylate) (PEA) induces the formation of biomimetic fibronectin (FN) (nano)networks upon simple adsorption from solutions, a process referred to as material-driven FN fibrillogenesis. The ability of PEA to organize FN has been demonstrated in 2D and 2.5D environments, but not as yet in 3D scaffolds, which incorporate three-dimensionality and chemical crosslinkers that may influence its fibrillogenic potential. In this paper we show for the first time that while three-dimensionality does not interfere with PEA-induced FN fibrillogenesis, crosslinking does, and we determined the maximum amount of crosslinker that can be added to PEA to maintain FN fibrillogenesis. For this, we synthesised 2D substrates with different amounts of crosslinker (1–10% of ethylene glycol dimethacrylate) and studied the role of crosslinking in FN organization using AFM. The glass transition temperature was seen to increase with crosslinking density and, accordingly, polymer segmental mobility was reduced. The organization of FN after adsorption (formation of FN fibrils) and the availability of the FN cell-binding domain were found to be dependent on crosslinking density. Surface mobility was identified as a key parameter for FN supramolecular organization. PEA networks with up to 2% crosslinker organize the FN in a similar way to non-crosslinked PEA. Scaffolds prepared with 2% crosslinker also had FN (nano)networks assembled on their walls, showing PEA’s ability to induce FN fibrillogenesis in 3D environments as long as the amounts of crosslinker is low enough.
Databáze: OpenAIRE
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