Effect of pH and Temperature on Bacillus sp. R2 Chitinase Activity and Stability
| Autor: | Ahmad Rafik EL-Mahdy, Mohamad Hisham EL-Massry, Ben Amar Cheba, Taha I. Zaghloul |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Initial activity Chitinase activity Bacillus sp 03 medical and health sciences chemistry.chemical_compound Hydrolysis Chitin characterization Bacillus sp. R2 General Environmental Science chemistry.chemical_classification Chromatography biology pH Chemistry activity temperature Heat stability stability 030104 developmental biology Enzyme Biochemistry chitinase Chitinase biology.protein General Earth and Planetary Sciences |
| Zdroj: | Procedia Technology. 22:471-477 |
| ISSN: | 2212-0173 |
| Popis: | The purified chitinase of Bacillus sp. R2 showed a molecular weight of 41.68 KDa, an optimal pH and temperature of 7.5 and 40 °C respectively. The enzyme was stable between pH 7 to 8 but it lost 57% and 62% of its initial activity at the pH 6 and 9 respectively. The purified chitinase also exhibited complete heat stability for 1 h at 40 °C and retained above 50% and 30% of its original activity after 30 min heating at 50 °C and 60 °C respectively. The chitinase activation energy for chitin hydrolysis was 12.93 Kcal/mol. To the best of our knowledge, this is the first study about marine chitinase characterization from novel gram variable Bacillus isolated from the Red sea. |
| Databáze: | OpenAIRE |
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